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Abstract

The fungi are a group of organisms that lack chlorophyll, reproduce by the production of spores (sexually and/or asexually), and contain chitin or cellulose in their cell walls. In contrast to bacteria, they are eukaryotic and have cell membranes that are rich in sterols, i.e., ergosterol. They are not susceptible to usual antibacterial antimicrobials but are to polyene antimicrobials that have a marked affinity for sterols.

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References

References

  1. Adams, E. D., Jr., and Cooper, B. H. Evaluation of a modified Wickerham medium for identification of medically important yeasts. Am. J. Med. Technol. 40: 377, 1974.

    PubMed  Google Scholar 

  2. Ajello, L., and Georg, L. K. In vitrocultures for differentiating between atypical isolates of Trichophyton mentagrophytesand Trichophyton rubrum. Mycol. Appl.8: 3, 1957.

    Article  CAS  Google Scholar 

  3. Bardana, E. J., Gerber, J. D., Craig, S., and Cianiulli, F. D. The general and specific humoral immune response to pulmonary aspergillosis. Am. Rev. Resp. Dis.112: 799, 1975.

    PubMed  Google Scholar 

  4. Block, E. R., Jennings, A. E., and Bennett, J. E. Variables influencing suscepti-bility testing of Cryptococcus neoformans to 5-fluorocytosine. Antimicrob. Agents Chemother.4: 392, 1973.

    PubMed  CAS  Google Scholar 

  5. Campbell, M. J., and Clayton, Y. M. Bronchopulmonary aspergillosis. Am. Rev. Resp. Dis.89: 186, 1964.

    PubMed  CAS  Google Scholar 

  6. Casals, J. B. Tablet sensitivity testing of pathogenic fungi. J. Clin. Pathol.32: 719, 1979.

    Article  PubMed  CAS  Google Scholar 

  7. Ellis, N. S., Bartlett, M. D., and Smith, J. W. Assay for yeast susceptibility to 5-fluorocytosine and amphotericin B in a frozen micro titer system. Am. J. Clin. Pathol.72: 194, 1979.

    PubMed  CAS  Google Scholar 

  8. Fleming, W. H., III, Hopkins, J. M., and Land, G. A. New culture medium for the presumptive identification of Candida albicans and Cryptococcus neoformans. J. Clin. Microbiol.5: 236, 1977.

    PubMed  CAS  Google Scholar 

  9. Gerber, J. D., and Jones, R. D. Immunologic significance of Aspergillus antigens of six species of Aspergillus in the serodiagnosis of aspergillosis. Am. Rev. Resp. Dis.108: 1124, 1973.

    PubMed  CAS  Google Scholar 

  10. Goldberg, L., Kozinn, P. J., Wise, G. J., Nouri, N., and Brooks, R. B. Incidence and significance of candiduria. J. Am. Med. Asso. 241: 582, 1979.

    Article  CAS  Google Scholar 

  11. Hopfer, R. L., Mills, K., Groschel, D. Radiometric method for determining the susceptibility of yeasts to 5-fluorocytosine. Antimicrob. Agents Chemother. 15: 313, 1979.

    PubMed  CAS  Google Scholar 

  12. Hopkins, J. M., and Land, G. A. Rapid method for determining nitrate utiliza-tion by yeasts. J. Clin. Microbiol.5: 497, 1977.

    PubMed  CAS  Google Scholar 

  13. Kaufman, C. S., and Merz, W. G. Two rapid pigmentation tests for the identifi-cation of Cryptococcus neoformans. J. Clin. Microbiol.15: 339, 1982.

    Google Scholar 

  14. Kaufman, L., and Blumer, S. Value and interpretation of serological tests for the diagnosis of cryptococcosis. Appl. Microbiol.16: 1907, 1968.

    PubMed  CAS  Google Scholar 

  15. Kaufman, L., McLaughlin, D. W., Clarke, M. J., and Blumer, S. Specific immu-nodiffusion test for blastomycosis. Appl. Microbiol. 26: 244, 1973.

    PubMed  CAS  Google Scholar 

  16. Kaufman, L., and Standard, P. Improved version of the exoantigen test for identification of Coccidioides immitis and Histoplasma capsulatum cultures. J. Clin. Microbiol. 8: 42, 1978.

    PubMed  CAS  Google Scholar 

  17. Land, G. A., Vinton, E. C., Adcock, G. B., and Hopkins, J. M. Improved auxanographic method for yeast assimilations: A comparison with other ap-proaches. J. Clin. Microbiol.2: 206, 1975.

    PubMed  CAS  Google Scholar 

  18. Mazens, M. F., Andrews, G. P., and Bartlett, R. C. Comparison of microdilution and broth dilution techniques for the susceptibility testing of yeasts to 5- fluorocytosine and amphotericin B. Antimicrob. Agents Chemother.15: 475, 1979.

    PubMed  CAS  Google Scholar 

  19. McDearman, S. C., and Young, J. M. The development of positive serologic tests with Histoplasma capsulatum antigens following single histoplasmin skin tests. Am. J. Clin. Pathol.34: 434, 1960.

    Google Scholar 

  20. Merz, W. G., Evans, G. L., Shadomy, S., Anderson, S., Kaufman, L., Kozinn, P. J., MacKenzie, D. W. R., Protzman, W. P., and Remington, J. S. Laboratory evaluation of serological tests for systemic candidiasis: A cooperative study. J.Clin. Microbiol. 5: 596, 1977.

    PubMed  CAS  Google Scholar 

  21. Mickelsen, P. A., McCarthy, L. A., and Propst, M. A. Further modifications of the auxanographic method for identification of yeasts. J. Clin. Microbiol. 5: 297, 1977.

    CAS  Google Scholar 

  22. Murray, P. R., Van Scoy, R. E., and Roberts, G. D. Should yeasts in respiratory secretions be identified? Mayo Clin. Proc. 52: 42, 1977.

    PubMed  CAS  Google Scholar 

  23. Nielsen, H. S. Biological properties of skin test antigens of yeast from Sporotrichum schenckii. J. Infect. Dis. 118: 173, 1968.

    Article  Google Scholar 

  24. Odds, F. C. Candida and Candidosis. Baltimore, MD, University Park Press, 1979, p. 55.

    Google Scholar 

  25. Paliwal, D. K., and Randhawa, H. S. Evaluation of a simplified Guizotia abyssi- nicaseed medium for differentiation of Cryptococcus neoformans. J. Clin. Microbiol. 7: 346, 1978.

    CAS  Google Scholar 

  26. Palmer, D. F., Kaufman, L., Kaplan, W., and Calvallaro, J. J. Serodiagnosis of Mycotic Diseases. Springfield, IL, Charles C Thomas, 1977.

    Google Scholar 

  27. Philpot, C. The differentiation of Trichophyton mentagrophytesfrom T. rub- rumby a simple urease test. Sabouraudia 5: 189, 1967.

    Article  PubMed  CAS  Google Scholar 

  28. Prevost, E., Newell, R.: Commercial cryptococcal latex kit: Clinical evaluation in a medical center hospital. J. Clin. Microbiol. 8: 529, 1978.

    PubMed  CAS  Google Scholar 

  29. Rhodes, J. C., and Roberts, G. D. Comparison of four methods for determining nitrate utilization by cryptococci. J. Clin. Microbiol. 1: 9, 1975.

    PubMed  CAS  Google Scholar 

  30. Roberts, G. D. Laboratory diagnosis of fungal infections. Human Pathol. 7: 161, 1976.

    Article  CAS  Google Scholar 

  31. Roberts, G. D., Horstmeier, C. D., Land, G. A., and Foxworth, J. H. Rapid urea broth test for yeasts. J. Clin. Microbiol.7: 584, 1978.

    PubMed  CAS  Google Scholar 

  32. Roberts, G. D., and Larsh, H. W. The serologic diagnosis of extracutaneous sporotrichosis. Am. J. Clin. Pathol.56: 597, 1971.

    PubMed  CAS  Google Scholar 

  33. Roberts, R. C., Wenzel, F. J., and Emanuel, D. A. Precipitating antibodies in a midwest dairy farming population toward the antigens associated with farmer’s lung disease. J. Allerg. Clin. Immunol.57: 518, 1976.

    Article  CAS  Google Scholar 

  34. Segal, E., and Ajello, L. Evaluation of a new system for the rapid identification of clinically important yeasts. J. Clin. Microbiol.4: 157, 1976.

    PubMed  CAS  Google Scholar 

  35. Shadomy, S. In vitro studies with 5-fluorocytosine. App. Microbiol17:871, 1969.

    CAS  Google Scholar 

  36. Shadomy, S. Further in vitrostudies with 5-fluorocytosine. Infect. Immunol2: 484, 1970.

    CAS  Google Scholar 

  37. Smith, C. D., and Goodman, N. L. Improved culture method for the isolation of Histoplasma capsulatumand Blastomyces dermatitidisfrom contaminated specimens. Am. J. Clin. Pathol63: 276, 1975.

    PubMed  CAS  Google Scholar 

  38. Stockman, L., and Roberts, G. D. Specificity of the latex test for cryptococcal antigen: a rapid, simple method for eliminating interference factors. J. Clin. Microbiol17: 945, 1983.

    PubMed  CAS  Google Scholar 

  39. Standard, P. G., and Kaufman, L. Specific immunological test for the rapid identification of members of the genus Histoplasma. J. Clin. Microbiol 3:191,

    Google Scholar 

  40. Standard, P. G., and Kaufman, L. Immunological procedure for the rapid and specific identification of Coccidioides immitis. J. Clin. Microbiol 5:149,

    Google Scholar 

  41. Standard, P. G., and Kaufman, L. Manual for the immunological identification of pathogenic fungus cultures. U.S. Department of Health, Education and Welfare, Public Health Service, Center for Disease Control, 1979.

    Google Scholar 

  42. Strimlan, C. V., Dines, D. E., Rogers-Sullivan, R. F., Roberts, G. D., and Sheehan, W. C. Respiratory tract aspergillus. Minn. Med.63: 25, 1980.

    PubMed  CAS  Google Scholar 

  43. Utz, C., and Shadomy, S. A diffusion disc susceptibility test for 5-fluorocytosine. In Williams, J. D., and Geddes, A. M. (eds.), Chemotherapy, Vol. 2. New York, Plenum, 1976, p. 81.

    Google Scholar 

  44. Utz, C. J., White, S., and Shadomy, S. New medium for in vitro susceptibility studies with amphotericin B. Antimicrob. Agents Chemother. 10: 776, 1976.

    CAS  Google Scholar 

  45. Weeks, R. J. A rapid simplified medium for converting the mycelial phase of Blastomyces dermatitidis to the yeast phase. Mycopathol. 22: 153, 1964.

    CAS  Google Scholar 

  46. Wolin, H. L., Bevis, M. L., and Laurora, N. An improved synthetic medium for the rapid production of chlamydospores by Candida albicans. Sabouraudia 2: 96, 1962.

    Article  Google Scholar 

  47. Young, R. C., and Bennett, J. C. Invasive aspergillosis absence of detectable antibody response. Am. Rev. Resp. Dis. 104: 710, 1971.

    PubMed  CAS  Google Scholar 

  48. Zimmer, B. L., and Roberts, G. D. Rapid selective urease test for presumptive identification of Cryptococcus neoformans. J. Clin. Microbiol. 10: 380, 1979.

    PubMed  CAS  Google Scholar 

General References for the Identification of Filamentous Fungi

  • Barnett, H. L., and Hunter, B. B. Illustrated Genera of Imperfect Fungi. Minneapolis, Burgess, 1972.

    Google Scholar 

  • Barron, G. L. The Genera of Hyphomycetes from Soil. Baltimore, Williams and Wilkins, 1968.

    Google Scholar 

  • Conant, N. F., Smith, D. T., Baker, R. D., and Callaway, J. L. Manual of Clinical Mycology. Philadelphia, Saunders, 1971.

    Google Scholar 

  • Emmons, C. W., Binford, C. H., Utz, J. P., and Kwon-Chung, K. J. Medical Mycology, 3rd ed. Philadelphia, Lea and Febiger, 1977.

    Google Scholar 

  • Gilman, J. C. A Manual of Soil Fungi, 2nd ed. Ames, Iowa State University Press, 1957.

    Google Scholar 

  • Koneman, E. W., Roberts, G. D., and Wright, S. F. Practical Laboratory Mycology. Baltimore, Williams and Wilkins, 1978.

    Google Scholar 

  • McGinnis, M. R. Laboratory Handbook of Medical Mycology. New York, Academic Press, 1980.

    Google Scholar 

  • Raper, K. B., and Fennell, D. I. The Genus Aspergillus. Baltimore, Williams and Wilkins, 1965.

    Google Scholar 

  • Rebell, G., and Taplin, D. Dermatophytes, Their Recognition and Identification. Coral Gables, FL, University of Miami Press, 1970.

    Google Scholar 

  • Wilson, J. W., and Plunkett, O. A. The Fungous Diseases of Man. Berkeley, Univer-sity of California Press, 1965.

    Google Scholar 

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Roberts, G.D. (1985). Fungi. In: Washington, J.A. (eds) Laboratory Procedures in Clinical Microbiology. Springer, New York, NY. https://doi.org/10.1007/978-1-4612-5070-8_7

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  • DOI: https://doi.org/10.1007/978-1-4612-5070-8_7

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