The raf Oncogene

  • U. R. Rapp
  • T. I. Bonner
  • J. L. Cleveland
Part of the Experimental Biology and Medicine book series (EBAM, volume 11)


v-raf is the oncogene of 3611 MSV and was isolated from mouse cells by transduction with murine leukemia virus (MuLV). v-raf is a new member of the src family of oncogenes and has associated ser/thr specific protein kinase activity. The active cellular homolog, c-raf-1, maps on chromosome 6 in the mouse and on chromosome 3p25 in man. There is a second gene in man, c-raf-2, which is an inactive pseudogene. the v-raf as well as the c-raf coded proteins are located predominantly in the cytoplasm. The active human gene, c-raf-1, has a complex structure with 15 exons, 9 of which are homologous to v-raf. An avian carcinoma virus, MH2, transduces the avian homologue of v-raf, v-mil, in addition to a second oncogene, v-myc. v-mil shares sequences with 11 exons of c-raf-1. A 3’ portion of c-raf-1 containing six of the seven amino acid differences relative to v-raf can substitute for 3’ portion of v-raf in a transformation assay. Sequence homologies between c-raf-1 and Moloney leukemia virus at both ends of v-raf indicate that the viral gene was acquired by homologous recombination, probably at the DNA level.


Murine Leukemia Virus Small Cell Lung Carcinoma Helper Virus Hemopoietic Cell Specific Protein Kinase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© The Humana Press Inc. 1985

Authors and Affiliations

  • U. R. Rapp
    • 1
  • T. I. Bonner
    • 2
  • J. L. Cleveland
    • 1
  1. 1.Laboratory of Viral CarcinogenesisNational Cancer InstituteFrederickUSA
  2. 2.Laboratory of Cell BiologyNational Institute of Mental HealthBethesdaUSA

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