Purification and Characterization of a High Molecular Weight Human Milk Ribonuclease: Its Potential Use for Diagnosis and Prognosis of Human Mammary Neoplasia

Hemavathj, R.; Das, M. R.

doi:10.1007/978-1-4612-5008-1_37

R. Hemavathj⁴ &
M. R. Das⁴

Part of the book series: Experimental Biology and Medicine ((EBAM,volume 11))

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Abstract

A high molecular weight ribonuclease (MW 80,000 daltons) from human milk (HMRNase) has been isolated and purified to homogeneity. Antiserum raised against this enzyme does not cross react with any of the human serum proteins, suggesting that the enzyme is synthesized in the mammary cells and secreted into the milk. The anti HMRNase antibody is expected to be a useful reagent for diagnostic and prognostic studies of human mammary neoplasia in view of the reverse correlation of the amount of ribonuclease present in human milk and mammary tumour incidence implicated in earlier studies (1).

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Authors and Affiliations

Centre for Cellular & Molecular Biology, Hyderabad, 500007, India
R. Hemavathj & M. R. Das

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R. Hemavathj
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M. R. Das
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Editors and Affiliations

National Institutes of Health, Bethesda, Maryland, USA
Robert C. Gallo
Institut Pasteur, Lille, France
Dominique Stehelin
Istituto di Microbiologia, University of Genova, Genova, Italia
Oliviero E. Varnier

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Hemavathj, R., Das, M.R. (1985). Purification and Characterization of a High Molecular Weight Human Milk Ribonuclease: Its Potential Use for Diagnosis and Prognosis of Human Mammary Neoplasia. In: Gallo, R.C., Stehelin, D., Varnier, O.E. (eds) International Symposium: Retroviruses and Human Pathology. Experimental Biology and Medicine, vol 11. Humana Press. https://doi.org/10.1007/978-1-4612-5008-1_37

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DOI: https://doi.org/10.1007/978-1-4612-5008-1_37
Publisher Name: Humana Press
Print ISBN: 978-1-4612-9396-5
Online ISBN: 978-1-4612-5008-1
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