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Response to an Active Vitamin D3 Metabolite of Transplantable Human Myeloid Leukemic Cell Lines in Adult Nude Mice

  • Conference paper
Experimental Hematology Today—1985

Part of the book series: Experimental Hematology Today ((HEMATOLOGY,volume 1985))

  • 31 Accesses

Abstract

Tumorigenicity of human myeloid leukemic cell lines KG-1, U937, HL-60 and K562 was studied in adult cyclophosphamide-primed nude mice (CPNM). All inocula except KG-1 consistently grew nonmet-astatic subcutaneous (sc) tumors after 3 weeks. Cytologic, immuno- and enzyme-cytochemical tests proved identity of respective tumor and cultured cells. Adenosine triphosphatase (ATPase) reactivity was limited to tumorigenie cells in vivo, while leucine aminopeptidase (LAP) was present both in cultured and tumor cells. Isoenzyme electrophoretic patterns clearly delineated each cell line, establishing clonality and confirming tumor cell origin. Antileukemic activity of an active vitamin D3 metabolite (D3M), 1,25-(0H)2D3, was assessed against leukemic sarcomas in vivo By transplantation of U937, HL-60 and K562 cells, and concomitant contralateral implantation of mini-osmotic pumps (MOP) containing 103ng/ml D3M in propylene glycol (PG). Tumor inhibition occurred in 75% of treated CPNM bearing HL-60, 50% with K562 and none with U937. Tumors proliferated in all untreated and control (MOP + PG) mice, but sarcomas in treated mice developed over 1 week later than in the others. Control and tumor- bearing D3M-treated mice died earlier than untreated mice, or treated mice without tumors, relating coincidence of tumor and PG, possibly through a metabolite of PG or a product of PG with the MOP. Tumor load was not a factor in lifespans of treated mice. Subclones of each line showed resistance to D3M activity in vivo. CPNM are useful models for study of human myeloid leukemic cells in vivo. MOP implants deliver consistent doses of antineoplastic agents and their solvents in vivo. D3M in an appropriate vehicle may be a valuable adjunctive agent for antineoplastic therapy of human myeloid leukemias.

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Author information

Authors and Affiliations

  1. Laboratory of Developmental Hematopoiesis, USA

    Gene K. Potter & Malcolm A. S. Moore

  2. Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, Box 543, New York City, New York, 10021, USA

    Gene K. Potter

  3. Division of Neuro-Oncology, USA

    Anwar N. Mohamed

  4. Human Cancer Serology Laboratory, USA

    Nicholas C. Dracopoli

  5. Biostatistics Laboratory, Sloan-Kettering Institute for Cancer Research, New York, New York, USA

    Susan L. B. Groshen

  6. Department of Radiation Oncology, Indiana University Hospital, Indiana University School of Medicine, Indianapolis, Indiana, USA

    Rong Nian Shen

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  1. Gene K. Potter
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  2. Anwar N. Mohamed
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  3. Nicholas C. Dracopoli
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  4. Susan L. B. Groshen
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  5. Rong Nian Shen
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  6. Malcolm A. S. Moore
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Editor information

Editors and Affiliations

  1. Physiology Department, Uniformed Services University of the Health Sciences, 20814, Bethesda, MD, USA

    S. J. Baum

  2. Laboratory of Microbiology and Immunology, National Institute of Dental Research, NIH, 29782, Bethesda, MD, USA

    Dov H. Pluznik

  3. Department of Life Sciences, Bar-Ilan University, Ramat-Gan, Israel

    L. A. Rozenszajn

  4. Department of Medical Laboratories, Meir Hospital, Kfar-Sava, Israel

    L. A. Rozenszajn

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© 1986 Springer-Verlag New York Inc.

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Potter, G.K., Mohamed, A.N., Dracopoli, N.C., Groshen, S.L.B., Shen, R.N., Moore, M.A.S. (1986). Response to an Active Vitamin D3 Metabolite of Transplantable Human Myeloid Leukemic Cell Lines in Adult Nude Mice. In: Baum, S.J., Pluznik, D.H., Rozenszajn, L.A. (eds) Experimental Hematology Today—1985. Experimental Hematology Today, vol 1985. Springer, New York, NY. https://doi.org/10.1007/978-1-4612-4920-7_17

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  • DOI: https://doi.org/10.1007/978-1-4612-4920-7_17

  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-0-387-96273-3

  • Online ISBN: 978-1-4612-4920-7

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