Abstract
Considerable progress has been made in the last few years in the development of techniques to produce and analyze mutations in Escherichia coli ribosomal RNA. It is now possible to introduce mutations into any site in the 16S, 23S, and 5S rRNAs, and a number of techniques are available to analyze structural and functional effects of these mutations. The development of this genetic system comes at a time when rapid progress has also occurred in the biochemical and comparative phylogenetic studies of rRNA. We now know a great deal about the structure of the rRNAs and have some evidence for functionally important sites as well. The introduction of site-directed mutagenic techniques promises to be particularly useful in further defining the structure and function of these and other specific regions of rRNA. In this chapter I will describe the contributions from our laboratory (and our collaborators) to the area of site-directed mutagenesis of rRNA. It represents the considerable effort of a number of very fine co-workers.
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Dahlberg, A.E. (1986). Site-Directed Mutagenesis of Escherichia coli Ribosomal RNA. In: Hardesty, B., Kramer, G. (eds) Structure, Function, and Genetics of Ribosomes. Springer Series in Molecular Biology. Springer, New York, NY. https://doi.org/10.1007/978-1-4612-4884-2_40
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DOI: https://doi.org/10.1007/978-1-4612-4884-2_40
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