Abstract
“Translational initiation” consists of an ill-defined number of interrelated steps preceding the formation of the first peptide bond. In Escherichia coli, in addition to mRNA, fmet-tRNA, and ribosomal subunits, initiation involves and requires three factors (IF-1, IF-2, and IF-3) and a GTP molecule. Fundamental problems concerning how the mRNA initiation region and the initiator tRNA are recognized, the role played by the initiation factors and GTP, and important mechanistic aspects, including the mechanism by which the factors are ejected from ribosomes, still remain open.
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Gualerzi, C.O., Pon, C.L., Pawlik, R.T., Canonaco, M.A., Paci, M., Wintermeyer, W. (1986). Role of the Initiation Factors in Escherichia coli Translational Initiation. In: Hardesty, B., Kramer, G. (eds) Structure, Function, and Genetics of Ribosomes. Springer Series in Molecular Biology. Springer, New York, NY. https://doi.org/10.1007/978-1-4612-4884-2_36
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DOI: https://doi.org/10.1007/978-1-4612-4884-2_36
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