Stereochemical Analysis of Ribosomal Transpeptidation, Translocation, and Nascent Peptide Folding
Ribosomal translation includes three main reactions: codon-dependent binding of aminoacyl-tRNA, transpeptidation, and translocation (Fig. 32.1). Cotranslational folding of a nascent peptide also takes place on the ribosome. None of these processes have been studied on the detailed molecular level in terms of molecular kinematics and concrete atomic interactions. At the same time, the atomic structure of tRNA, the principles of tRNA-codon interactions, the chemistry of transpeptidation reactions, and certain restrictions imposed by the ribosome are known. Using this knowledge and accepting some reasonable postulates, the ribosomal processes can be studied by means of molecular models. Some results of the stereochemical modeling experiments are briefly given below.
KeywordsCodon Lactate Amide Proline Carbonyl
Unable to display preview. Download preview PDF.
- Rich, A. (1974). How transfer RNA may move inside the ribosome. In: Ribosomes, eds. Nomura, M., et al. Cold Spring Harbor Laboratory, New York, pp. 871–884.Google Scholar
- Sundaralingam, M., Brennan, T., Yathindra, N., Ichikawa, T. (1975). Stereochemistry of messenger RNA (codon) — transfer RNA (anticodon) interaction on the ribosome during peptide bond formation. In: Structure and conformation of nucleic acids and protein — nucleic acid interactions, eds. Sundaralingam, M., Rao, S.T. University Park Press, Baltimore, pp. 101–115.Google Scholar