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Immuno Electron Microscopy on Escherichia coli Ribosomes

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Structure, Function, and Genetics of Ribosomes

Part of the book series: Springer Series in Molecular Biology ((SSMOL))

Abstract

Immuno electron microscopy (IEM), one of several techniques that have proved useful for the elucidation of the structural organization of the ribosome, has provided considerable information on the topography of the proteins on the ribosomal surface. In 1973, the first ribosomal proteins were localized on the surface of the 50S subunit of Escherichia coli by IEM in our laboratory (Wabl, 1973; Wabl, 1974). Since then, we have continued this work (Tischendorf et al., 1974a, b), and since 1974, results on the localization of ribosomal components have also been forthcoming from other research groups (Lake etal., 1974; Boublik etal., 1976). In this article the results obtained on the topography of the ribosomal proteins of E. coli by IEM will be described, and they will be compared with the results obtained from other methods, such as neutron-scattering, crosslinking, and energy transfer measurements.

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Authors
  1. G. Stöffler
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  2. M. Stöffler-Meilicke
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Editors and Affiliations

  1. Clayton Foundation Biochemical Institute Department of Chemistry, The University of Texas at Austin, 78712, Austin, Texas, USA

    Boyd Hardesty  & Gisela Kramer  & 

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Stöffler, G., Stöffler-Meilicke, M. (1986). Immuno Electron Microscopy on Escherichia coli Ribosomes. In: Hardesty, B., Kramer, G. (eds) Structure, Function, and Genetics of Ribosomes. Springer Series in Molecular Biology. Springer, New York, NY. https://doi.org/10.1007/978-1-4612-4884-2_2

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  • DOI: https://doi.org/10.1007/978-1-4612-4884-2_2

  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4612-9346-0

  • Online ISBN: 978-1-4612-4884-2

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