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Study of the Antigenic Profile of Normal Myelo-Monocytic Progenitors and Leukemic Cell Lines Using Monoclonal Antibodies

  • M. C. Alonso
  • R. Solana
  • A. Torres
  • R. Ramirez
  • C. Navarrete
  • J. Pena
  • H. Festenstein

Abstract

Studies of hematopoietic differentiation have classically relied on the ability to identify mature hematopoietic cells and their precursors by morphological, histochemical, and functional criteria. However, the study of early myeloid differentiation has been difficult due to the small number of progenitor cells and their lack of distinctive features (1). To further characterize these progenitors, proliferative assays in the presence of specific growth factors have been applied (2,3). With the advent of monoclonal antibody (mAb) technology, it has been possible to define surface membrane markers which discriminate between subpopulations of lymphoid and myeloid cells and which relate to both the ontogeny and functional heterogeneity of these subpopulations. With reference to the myelo-monocytic lineage, the efforts of several laboratories have resulted in the development of mAbs that distinguish markers shared by myeloid cells and cells of other lineage (4), other markers specific for the myelo-monocytic series (5), and finally markers selective for fully differentiated monocytes (6). These mAbs have made it possible to associate membrane structural features with particular stages of normal myeloid differentiation and with leukemic cells that represent a malignant proliferation of a given stage of maturation (7,8).

Keywords

Bone Marrow Cell K562 Cell Line Myeloid Cell Line Hematopoietic Differentiation Antigenic Profile 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag New York Inc. 1986

Authors and Affiliations

  • M. C. Alonso
  • R. Solana
  • A. Torres
  • R. Ramirez
  • C. Navarrete
  • J. Pena
  • H. Festenstein

There are no affiliations available

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