TPA-Induced Modulation of B Cell Differentiation Antigens Defined by Monoclonal Antibodies (HD6, HD28, HD37, HD39)
B cell neoplasms, particularly chronic lymphocytic leukemias (CLL) and non-Hodgkin’s lymphomas, can be considered as monoclonal cell populations which are arrested at certain stages of normal B cell differentiation. Efforts to suspend this restriction by treatment with the tumor promoter 12-O-tetradecanoylphorbol 13-acetate (TPA) resulted in the stimulation of terminal differentiation-like changes of the leukemic cells (1). This process was assessed by alterations in cytoplasmic and surface immunoglobulin content (2), HLA-class II antigens (3), complement receptor (4), and morphological features (5). Lately, modulations of cell surface structures after TPA stimulation, which were followed up by monoclonal antibodies specific for B cell differentiation antigens, supported the assumption that chronic lymphocytic leukemias can be induced by TPA to further maturation towards the plasma cell stage (6,7). Thus, the monitoring of TPA-induced alterations of differentiation antigens by B cell-specific antibodies could help to i) describe more precisely the reaction pattern of the respective monoclonal antibody, ii) define closer sections along the normal B cell differentiation pathway, and iii) gain information about the genetic program of the various leukemia and lymphoma types.
KeywordsGlycerol Filtration Lymphoma Leukemia Leucine
Unable to display preview. Download preview PDF.
- 5.Pantazis, P., N. Pavlidis, G.E. Demetrakopoulos, and J.E. Dahlberg. 1982. Morphological changes in cultured human leukemic cells (K 562) treated with the tumor promotor 12-0-tetradecanoylphorbol-13-acetate. Biol. Cell. 46: 143.Google Scholar
- 8.Lennert, K. 1978. Handbuch der speziellen pathologischen Anatomie und Histologic. Malignant lymphomas. Springer-Verlag, Berlin.Google Scholar
- 9.Morich, F.-J., F. Momburg, G. Moldenhauer, K.-U. Hartmann, and K.J. Bross. 1983. Immunoperoxidase slide assay (IPSA)—a new screening method for hybridoma supernatants directed against cell surface antigens compared to other binding assays. Immunobiol. 164: 192.Google Scholar