TPA-Induced Modulation of B Cell Differentiation Antigens Defined by Monoclonal Antibodies (HD6, HD28, HD37, HD39)

  • Reinhard Schwartz
  • Gerhard Moldenhauer
  • Bernd Dörken
  • Antonio Pezzutto
  • Frank Momburg
  • Volker Schirrmacher

Abstract

B cell neoplasms, particularly chronic lymphocytic leukemias (CLL) and non-Hodgkin’s lymphomas, can be considered as monoclonal cell populations which are arrested at certain stages of normal B cell differentiation. Efforts to suspend this restriction by treatment with the tumor promoter 12-O-tetradecanoylphorbol 13-acetate (TPA) resulted in the stimulation of terminal differentiation-like changes of the leukemic cells (1). This process was assessed by alterations in cytoplasmic and surface immunoglobulin content (2), HLA-class II antigens (3), complement receptor (4), and morphological features (5). Lately, modulations of cell surface structures after TPA stimulation, which were followed up by monoclonal antibodies specific for B cell differentiation antigens, supported the assumption that chronic lymphocytic leukemias can be induced by TPA to further maturation towards the plasma cell stage (6,7). Thus, the monitoring of TPA-induced alterations of differentiation antigens by B cell-specific antibodies could help to i) describe more precisely the reaction pattern of the respective monoclonal antibody, ii) define closer sections along the normal B cell differentiation pathway, and iii) gain information about the genetic program of the various leukemia and lymphoma types.

Keywords

Glycerol Filtration Lymphoma Leukemia Leucine 

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Copyright information

© Spriger-Verlag New York Inc. 1986

Authors and Affiliations

  • Reinhard Schwartz
  • Gerhard Moldenhauer
  • Bernd Dörken
  • Antonio Pezzutto
  • Frank Momburg
  • Volker Schirrmacher

There are no affiliations available

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