TPA-Induced Modulation of B Cell Differentiation Antigens Defined by Monoclonal Antibodies (HD6, HD28, HD37, HD39)

  • Reinhard Schwartz
  • Gerhard Moldenhauer
  • Bernd Dörken
  • Antonio Pezzutto
  • Frank Momburg
  • Volker Schirrmacher


B cell neoplasms, particularly chronic lymphocytic leukemias (CLL) and non-Hodgkin’s lymphomas, can be considered as monoclonal cell populations which are arrested at certain stages of normal B cell differentiation. Efforts to suspend this restriction by treatment with the tumor promoter 12-O-tetradecanoylphorbol 13-acetate (TPA) resulted in the stimulation of terminal differentiation-like changes of the leukemic cells (1). This process was assessed by alterations in cytoplasmic and surface immunoglobulin content (2), HLA-class II antigens (3), complement receptor (4), and morphological features (5). Lately, modulations of cell surface structures after TPA stimulation, which were followed up by monoclonal antibodies specific for B cell differentiation antigens, supported the assumption that chronic lymphocytic leukemias can be induced by TPA to further maturation towards the plasma cell stage (6,7). Thus, the monitoring of TPA-induced alterations of differentiation antigens by B cell-specific antibodies could help to i) describe more precisely the reaction pattern of the respective monoclonal antibody, ii) define closer sections along the normal B cell differentiation pathway, and iii) gain information about the genetic program of the various leukemia and lymphoma types.


Chronic Lymphocytic Leukemia Reaction Pattern Hairy Cell Leukemia Blast Crisis Chronic Lymphocytic Leukemia Cell 
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  1. 1.
    Tötterman, T.H., K. Nilsson, and C. Sundstrom. 1980. Phorbol ester-induced differentiation of chronic lymphocytic leukemia cells. Nature 288: 176.PubMedCrossRefGoogle Scholar
  2. 2.
    Sugawara, I. 1982. The immunoglobulin production of human peripheral B lymphocytes induced by phorbol myristate acetate. Cell. Immunol. 72: 88.PubMedCrossRefGoogle Scholar
  3. 3.
    Okamura, J., M. Letarte, L.D. Stein, N.H. Sigal, and E.W. Gelfand. 1982. Modulation of chronic lymphocytic leukemia cells by phorbol ester: increase in la expression, IgM secretion and MLR stimulatory capacity. J. Immunol. 128: 2276.PubMedGoogle Scholar
  4. 4.
    Lindsten, T., C.B. Thompson, F.D. Finkelman, B. Andersson, and I. Scher. 1984. Changes in the expression of B cell surface markers on complement receptor-positive and complement receptor-negative B cells induced by phorbol myristate acetate. J. Immunol. 132: 235.PubMedGoogle Scholar
  5. 5.
    Pantazis, P., N. Pavlidis, G.E. Demetrakopoulos, and J.E. Dahlberg. 1982. Morphological changes in cultured human leukemic cells (K 562) treated with the tumor promotor 12-0-tetradecanoylphorbol-13-acetate. Biol. Cell. 46: 143.Google Scholar
  6. 6.
    LeBien, T.W., F.J. Bollum, W.G. Yasmineh, and J.H. Kersey. 1982. Phorbol ester-induced differentiation of a non-T, non-B leukemic cell line: model for human lymphoid progenitor cell development. J. Immunol. 128: 1316.PubMedGoogle Scholar
  7. 7.
    Gordon, J., H. Mellstedt, P. Aman, P. Biberfeld, and G. Klein. 1984. Pheno- typic modulation of chronic lymphocytic leukemia cells by phorbol ester: induction of IgM secretion and changes in the expression of B cell-associated surface antigens. J. Immunol. 132: 541.PubMedGoogle Scholar
  8. 8.
    Lennert, K. 1978. Handbuch der speziellen pathologischen Anatomie und Histologic. Malignant lymphomas. Springer-Verlag, Berlin.Google Scholar
  9. 9.
    Morich, F.-J., F. Momburg, G. Moldenhauer, K.-U. Hartmann, and K.J. Bross. 1983. Immunoperoxidase slide assay (IPSA)—a new screening method for hybridoma supernatants directed against cell surface antigens compared to other binding assays. Immunobiol. 164: 192.Google Scholar
  10. 10.
    Nadler, L.M., P. Stashenko, J. Ritz, P. Hardy, J.M. Pesando, and S.F. Schlossman. 1981. A unique cell surface antigen identifying lymphoid malignancies of B cell origin. J. Clin. Invest. 67: 134.PubMedCrossRefGoogle Scholar
  11. 11.
    Stashenko, P., L.M. Nadler, R. Hardy, and S.F. Schlossman. 1981. Expression of cell surface markers after human B lymphocyte activation. Proc. Natl. Acad. Sci. U.S.A. 78: 3848.PubMedCrossRefGoogle Scholar

Copyright information

© Spriger-Verlag New York Inc. 1986

Authors and Affiliations

  • Reinhard Schwartz
  • Gerhard Moldenhauer
  • Bernd Dörken
  • Antonio Pezzutto
  • Frank Momburg
  • Volker Schirrmacher

There are no affiliations available

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