Screening of Workshop “B” Series Antibodies by Radioimmunobinding to Human Leukocyte Cell Lines and to Cells from Human Lymphoid Tumors
The fifty-two monoclonal antibodies of the Workshop B series were received in April, 1984. They were allowed to thaw and were held at 8°C for the period of study (April–August, 1984). Aliquots of the original samples were diluted 1:20 with RPM1 1640 culture medium containing 5% fetal calf serum (FCS), 5% horse serum (HS), and 0.02% sodium azide. The diluted antibodies were then tested for binding to a panel of cells from human lymphoid lines or from lymphoid tumors. The phenotypes of the cells forming the panel had already been determined but additional monoclonal antibodies of known specificities were included to act as positive or negative controls in the binding assays.
KeywordsAlbumin Lymphoma Leukemia Glycine Myeloma
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