Report to Second International Workshop on Human Leukocyte Differentiation Markers: Boston, 1984 Tissue Localization of B Cell and Leukemic Reagents (Special Studies)
B cells are most commonly typed with monoclonal antibodies with the use of fluorescent second antibodies applied to cell suspensions. Until recently the most useful reagents for this purpose have been those which identified Dr and CALLA antigens; other antibodies such as B1 and B2 (1) have become prominent since the last workshop on B cell markers but their absolute specificity for B cells in the context of tissue section analysis remains doubtful (2). For the purpose of tissue studies and to investigate the biology of the B cell it is of interest to identify B cell-specific antibodies by the criterion of non-cross-reactivity with tissue elements. The analogy with the well-known T cell markers and the elucidation, in consequence of their discovery, of the T cell antigen receptor should serve as a powerful stimulus in this endeavor.
KeywordsTissue Element Monocytic Leukemia Cell Series Cell Antigen Receptor Absolute Specificity
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- 2.Hancock, W.W., N. Kraft, and R.C. Atkins. 1984. Importance of tissue localization in characterization of monoclonal antibodies to human leucocyte antigens. In: Leucocyte typing, A. Bernard, L. Boumsell, J. Dausset, C. Milstein, and S.F. Schlossman, eds. Springer-Verlag, Berlin, Heidelberg, p. 475.Google Scholar