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Report to Second International Workshop on Human Leukocyte Differentiation Markers: Boston, 1984 Tissue Localization of B Cell and Leukemic Reagents (Special Studies)

  • Norbert Kraft
  • Peter S. Giddy
  • Wayne W. Hancock
  • Robert C. Atkins
Conference paper

Abstract

B cells are most commonly typed with monoclonal antibodies with the use of fluorescent second antibodies applied to cell suspensions. Until recently the most useful reagents for this purpose have been those which identified Dr and CALLA antigens; other antibodies such as B1 and B2 (1) have become prominent since the last workshop on B cell markers but their absolute specificity for B cells in the context of tissue section analysis remains doubtful (2). For the purpose of tissue studies and to investigate the biology of the B cell it is of interest to identify B cell-specific antibodies by the criterion of non-cross-reactivity with tissue elements. The analogy with the well-known T cell markers and the elucidation, in consequence of their discovery, of the T cell antigen receptor should serve as a powerful stimulus in this endeavor.

Keywords

Tissue Element Monocytic Leukemia Cell Series Cell Antigen Receptor Absolute Specificity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Nadler, L.M., P. Stashenko, R. Hardy, A. van Agthoven, C. Terhorst, and S.F. Schlossman. 1981. Characterization of a human B cell specific antigen (B2) distinct from Bl. J. Immunol. 126:1941.PubMedGoogle Scholar
  2. 2.
    Hancock, W.W., N. Kraft, and R.C. Atkins. 1984. Importance of tissue localization in characterization of monoclonal antibodies to human leucocyte antigens. In: Leucocyte typing, A. Bernard, L. Boumsell, J. Dausset, C. Milstein, and S.F. Schlossman, eds. Springer-Verlag, Berlin, Heidelberg, p. 475.Google Scholar
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    Hancock, W.W., G.J. Becker, and R.C. Atkins. 1982. A comparison of fixatives and immunohistochemical techniques for use with monoclonal antibodies. Am. J. Clin. Pathol. 78:825.PubMedGoogle Scholar
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    Bradley, T.R., G.R. Pilkington, M. Garson, G.S. Hodgson, and N. Kraft. 1982. Cell lines derived from a myelomonocytic leukaemia. Brit. J. Haematol. 51:595.CrossRefGoogle Scholar

Copyright information

© Springer-Verlag New York Inc. 1986

Authors and Affiliations

  • Norbert Kraft
  • Peter S. Giddy
  • Wayne W. Hancock
  • Robert C. Atkins

There are no affiliations available

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