Patch Clamp and Noise Analysis Studies of Neurotransmitter Receptors of Cultured Insect Neurones
Studies of insect neuronal cultures prepared from the brains of embryonic cockroaches have shown that the in vitro neurones possess α-bungarotoxin binding sites and are depolarised by acetylcholine (ACh) and approximately 60% of them are hyperpolarized by the application of gamma-aminobutyric acid (GABA) (Lees et al., 1983; Lees et al., 1984). These studies have now been extended using the patch-clamp technique to obtain more information about the ionic channels activated by these neurotransmitters. The technique has been used in both the ‘cell-attached’ mode, in which case the transmitters were added to the saline in the pipette, or in the ‘whole cell clamp’ mode, in which case current fluctuations resulting from pressure application of the transmitters were analysed.
KeywordsHepes Acetylcholine EGTA Barbiturate Muscimol
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