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Patch Clamp and Noise Analysis Studies of Neurotransmitter Receptors of Cultured Insect Neurones

  • David J. Beadle
  • Yves Pichon
  • Takeshi Shimahara
Part of the Experimental and Clinical Neuroscience book series (ECN)

Abstract

Studies of insect neuronal cultures prepared from the brains of embryonic cockroaches have shown that the in vitro neurones possess α-bungarotoxin binding sites and are depolarised by acetylcholine (ACh) and approximately 60% of them are hyperpolarized by the application of gamma-aminobutyric acid (GABA) (Lees et al., 1983; Lees et al., 1984). These studies have now been extended using the patch-clamp technique to obtain more information about the ionic channels activated by these neurotransmitters. The technique has been used in both the ‘cell-attached’ mode, in which case the transmitters were added to the saline in the pipette, or in the ‘whole cell clamp’ mode, in which case current fluctuations resulting from pressure application of the transmitters were analysed.

Keywords

Current Noise Single Channel Conductance Membrane Patch Lorentzian Curve Open Time Constant 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Beadle, D.J., Hicks, D. and Middleton, C. (1982) Fine structure of neurones from embryonic Periplaneta americana growing in long term culture. J. Neurocytol. 11, 611–626.PubMedCrossRefGoogle Scholar
  2. Hamil, O.P., Marty, A., Naher, E., Sakmann, B. and Sigworth, F.J. (1981) Improved patch-clamp techniques for high resolution current recording from cells and cell-free membrane patches. Pflugers Arch 391, 85–100.CrossRefGoogle Scholar
  3. Lees, G., Beadle, D.J. and Botham, R.P. (1983) Cholinergic receptors on cultured neurones from the central nervous system of embryonic cockroaches. Brain Res. 288, 49–59.PubMedCrossRefGoogle Scholar
  4. Lees, G., Beadle, D.J., Neumann, R. and Benson, J.A. (in press). Responses to GABA by isolated insect neuronal somata: pharmacology and modulation by a benzodiazepine and a barbiturate. Brain Res.Google Scholar
  5. Lees, G., Benson, J.A. and Beadle, D.J. (1984) Putative insect neurotransmitters evoke electrophysiological responses from embryonic cockroach neurones in primary culture. Soc. Neurosci. Abst. 10, 688.Google Scholar

Copyright information

© The Humana Press Inc. 1986

Authors and Affiliations

  • David J. Beadle
    • 1
    • 2
  • Yves Pichon
    • 1
    • 2
  • Takeshi Shimahara
    • 1
    • 2
  1. 1.School of Biological SciencesThames PolytechnicLondonUK
  2. 2.Laboratoire de Neurobiologie Cellulaire du CNRSGif-sur-YvetteFrance

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