Although only a decade has passed since Kohler and Milstein (1) described the first production of hybridomas, monoclonal antibodies (Mab) have become a ubiquitous tool in the hands of scientists from a wide array of interests and specialties. A number of commercially produced Mabs are available for a variety of purposes, but most researchers require “tailor-made” Mabs. Not all of these researchers have suitable facilities and/or experience in tissue culture; and, even in the hands of experts, precious Mab-producing hybridoma clones are often lost as a result of the perils and hazards of cell culture. The archenemies of all cell culture laboratories are mycoplasma, bacteria, yeast, and fungus contaminations. In the absence of suitable sterile facilities (e.g., laminar flow hood) and in the hands of the novice, these perils are prominent. In addition, at times (for reasons poorly understood), the newly fused cells undergo a few rounds of replication, but fail to multiply when attempts are made to expand the culture. Furthermore, despite careful cryopreserving protocols, there are often problems with reestablishing hybridoma cells in culture from frozen stocks.
KeywordsHydrate Cage Titration Syringe Pyruvate
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