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Interspecific Hybridomas

  • Chapter
Methods of Hybridoma Formation

Part of the book series: Contemporary Biomedicine ((CB,volume 7))

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Abstract

The first reports of the production of immortal immunoglobulin-secreting cell lines from the fusion of normal lymphocytes with myeloma cells involved human lymphocytes and a mouse myeloma cell line (1,2). The aim of these studies by Schwaber and Cohen was to define defects in human immunodeficiency states, rather than to produce human antibody of predetermined specificity. The ability of mouse-mouse hybridomas (3) to provide a straightforward route to immunological reagents that are unmatched in specificity and unlimited in quantity has relegated the pioneering studies on human-mouse hybrids to relative obscurity. However, there are many questions, such as the one examined by Schwaber and Cohen, that arc best approached by the fusion of lymphocytes from a species other than the mouse to a suitable myeloma partner. Because appropriate myeloma partners are available for only a few species, such fusions are usually interspecific in nature. Interspecific fusions present unique problems and unique opportunities and are the subject of this review.

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Authors
  1. John A. Sogn
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Editors and Affiliations

  1. Biotherapeutics, Inc., Franklin, Tennessee, USA

    Arie H. Bartal

  2. Hybridoma Laboratory, Northern Israel Oncology Center, Rambam Medical Center, Haifa, Israel

    Arie H. Bartal

  3. Sloan-Kettering Cancer Center, New York, New York, USA

    Yashar Hirshaut

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© 1987 The Humana Press Inc.

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Sogn, J.A. (1987). Interspecific Hybridomas. In: Bartal, A.H., Hirshaut, Y. (eds) Methods of Hybridoma Formation. Contemporary Biomedicine, vol 7. Humana Press. https://doi.org/10.1007/978-1-4612-4826-2_17

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  • DOI: https://doi.org/10.1007/978-1-4612-4826-2_17

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-4612-9179-4

  • Online ISBN: 978-1-4612-4826-2

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