Hormonal and Genetic Factors in Plant Sex Expression
The direct effect of phytohormones on sexual differentiation was studied in a culture of isolated embryos of hemp (Cannabis sativa, cultivar US-6).
The seeds were surface-sterilized in a weak solution of KMnO4 or in 0.1% MgCl2 for 15 and 5 minutes, respectively, then washed several times in sterile water and transferred to 5 to 6 layers of damp filter paper and placed in Petri dishes. The rigid bivalvular seed coats burst after 14 to 18 hours. The mature embryos were removed from the seeds and the cotyledons cut off. The embryos were transferred into sterile test tubes containing White’s agar which includes minerals, vitamins, and sucrose (White 1949; Butenko 1964). The growth conditions included lighting, 0.002 W/cm2; temperature, 20 to 22°C; and relative humidity, 80%. For the first 7 days the photoperiod was 16 hours, and thereafter 8 hours, until sex expression became apparent.
KeywordsSucrose Starch Corn Mercury Estrogen
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