Modality Specific Analgesia Produced by Intrathecal Anti-Substance P Antibody

  • P. W Nance
  • J. Sawynok
  • D. M. Nance
Conference paper

Abstract

Substance P (SP) is a putative primary afferent transmitter of nociceptive information [1]. Intrathecal injection of SP produces an increased sensitivity to heat [2,3] and pressure [4] in tests for nociceptive thresholds. Recently, SP was shown to be released from spinal cord by noxious pressure but not noxious thermal stimulation, while somatostatin (SST) was selectively released by noxious thermal stimulation [5] suggesting a modality specific role for these peptides in relation to pain. Previously, antibodies to SP (anti-SP) and SST (anti-SST) have been used to demonstrate the presence of SP and SST immunoreactive fibers in the dorsal horn of the spinal cord [6]. In this study we have used antibodies to SP and SST to determine whether a modality specific analgesia for these peptides could be demonstrated. The present experiments first tested the tail flick latency to heat and the paw withdrawal response to pressure in rats following intrathecal injections of anti SP, anti-SST or normal serum. In a second experiment, the behavioral effects of anti-SP serum were compared to normal serum in rats which were tested prior to injection.

Keywords

Cage Diaminobenzidine Sera 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Nicoll RA, Schenker C, Leeman SE (1980) Substance P as a transmitter candidate. Ann Rev Neurosci 3: 227–268PubMedCrossRefGoogle Scholar
  2. 2.
    Yasphal K, Wright OM, Henry JL (1982) Substance P reduces tail flick latency: implications for chronic pain syndromes. Pain 14: 155–167PubMedCrossRefGoogle Scholar
  3. 3.
    Moochhala SM, Sawynok J (1984) Hyperalgesia produced by intrathecal substance P and related peptides: desensitization and cross desensitization. Br J Pharmac 82: 381–388Google Scholar
  4. 4.
    Matsumara H, Sakurada T, Hara A, Sakurada S, Kisara K (1985) Characterization of the hyperalgesic effect induced by intrathecal injection of substance P. Neuropharmacology 24: 421–426CrossRefGoogle Scholar
  5. 5.
    Kuriashi Y, Hirota N, Sato Y, Hino Y, Satoh M, Takagi H (1985) Evidence that substance P and somatostatin transmit separate information related to pain in the spinal dorsal horn. Brain Research 325: 294–298CrossRefGoogle Scholar
  6. 6.
    Hökfelt T, Elde R, Johansson O, Luft R, Nilsson G, Arimura A (1976) Immunohistochemical evidence for separate opulations of somatostatincontaining and substance P-containing primary afferent neurons in the rat. Neuroscience 1: 131–136PubMedCrossRefGoogle Scholar
  7. 7.
    Chrubisak J, Meynadier J, Blond S, Scherpereel P, Ackerman E, Weinstock M, Bonath K, Cramer H, Wünsch E (1984) Somatostatin, a potent analgesic. Lancet ii: 1208–1209CrossRefGoogle Scholar

Copyright information

© Springer-Verlag New York Inc. 1987

Authors and Affiliations

  • P. W Nance
    • 1
  • J. Sawynok
    • 1
  • D. M. Nance
    • 1
  1. 1.Departments of Medicine, Pharmacology and AnatomyDalhousie UniversityHalifaxCanada

Personalised recommendations