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Structure and Post-Translational Processing of the Preprotachykinins

  • A. J. Harmar
  • A. R. Pierotti
  • A. Armstrong
  • K. Chapman
  • J. S. Chahl
  • J. Going
  • C. R. W. Edwards
  • R. Rosie
  • J. Pascall
Conference paper

Abstract

Substance P is synthesised from two precursors, α and β preprotachykinin (PPT), which are derived from a single gene by differential RNA splicing [1,2]. We have recently elucidated the cDNA sequence of human β-PPT. The human β-PPT polypeptide contains, in addition to a region encoding SP, the sequences of neurokinin A (NkA) and of neuropeptide K (NpK), an N-terminally extended form of NkA first isolated from porcine brain [3]. We are presently searching for novel fragments of α- and β-PPT which may exhibit biological activity. Rat brain contains a 5,500 Mol. Wt. form of NkA-like immunoreactivity which apparently contains antigenic determinants related to both SP and NkA. This peptide may represent an intermediate in the post-translational processing of β-PPT.

Keywords

Substantia Nigra Carcinoid Tumour Porcine Brain Signal Peptidase Cleavage Site Royal Edinburgh Hospital 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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Copyright information

© Springer-Verlag New York Inc. 1987

Authors and Affiliations

  • A. J. Harmar
    • 1
  • A. R. Pierotti
    • 1
  • A. Armstrong
    • 1
  • K. Chapman
    • 1
  • J. S. Chahl
    • 2
  • J. Going
    • 3
  • C. R. W. Edwards
    • 4
  • R. Rosie
    • 1
  • J. Pascall
    • 1
  1. 1.MRC Brain Metabolism UnitRoyal Edinburgh HospitalEdinburghUK
  2. 2.Faculty of MedicineUniversity of NewcastleAustralia
  3. 3.Department of PathologyUniversity Medical SchoolEdinburghUK
  4. 4.Department of MedicineWestern General HospitalEdinburghUK

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