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Autoradiographic Localization of Substance P Binding Sites in Guinea-Pig Respiratory Tract

  • D. J. Watkins
  • E. Burcher
Conference paper

Summary

Autoradiographic binding sites for [125I]-Bolton-Hunter labelled substance P (BHSP) were investigated in the guineapig respiratory tract. Dense binding was observed on the smooth muscle of primary bronchi, secondary bronchi and terminal bronchioles. In the trachea, specific binding sites for BHSP were seen densely clustered over the trachealis muscle. Specific binding of BHSP to guinea-pig lung membranes was displaced by SP > SK > NK, indicating binding to a P-type site. No binding of BHSP was observed to arterioles in the lung. The presence of BHSP binding sites on guinea-pig respiratory smooth muscle is in accordance with the bronchoconstrictor actions of SP.

Keywords

Bronchial Smooth Muscle Dense Binding Terminal Bronchiole Tachykinin Receptor Trachealis Muscle 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Hua X-Y, Theodorsson-Norheim E, Brodin E, Lundberg JM, Hokfelt T (1985) Multiple tachykinins (neurokinin A, neuropeptide K and substance P) in capsaicin-sensitive sensory neurons in the guinea-pig. Regul Pept 13:1–19.PubMedCrossRefGoogle Scholar
  2. 2.
    Ghatei MA, Sheppard MN, O’Shaughnessy DJ, Adrian TE, McGregor GP, Polak JM, Bloom SR (1982) Regulatory peptides in the mamalian respiratory tract. Endocrinology 111:1248–1254.PubMedCrossRefGoogle Scholar
  3. 3.
    Hua X, Lundberg JM, Theodorsson-Norheim E, Brodin E (1984) Comparison of cardiovascular and bronchoconstrictor effects of substance P, substance K and other tachykinins. Naunyn-Schmiedeberg’s Arch Pharmacol 328:196–201.CrossRefGoogle Scholar
  4. 4.
    Saria A, Lundberg JM, Skofitsch G, Lembeck F (1983) Vascular protein leakage in various tissues induced by substance P, capsaicin, bradykinin, serotonin, histamine and by antigen challenge. Naunyn-Schmiedeberg’s Arch Pharmaco1 324:212–218.CrossRefGoogle Scholar
  5. 5.
    Mazurek N, Pecht I, Teichberg VI, Blumberg S, (1981) The role of the N-terminal tetrapeptide in the histamine-releasing action of substance P. Neuropharmacology 20:1025–1027.PubMedCrossRefGoogle Scholar
  6. 6.
    Buck SH, Burcher E, Shults CW, Lovenberg W, O’Donohue TL (1984) Novel pharmacology of substance Kbinding sites: a third type of tachykinin receptor. Science 226:987–989.PubMedCrossRefGoogle Scholar
  7. 7.
    Burcher E, Buck SH, Lovenberg W, O’Donohue TL (1984) Characterization and auto radiographic localization of multiple tachykinin binding sites in gastrointestinal tract and bladder. J Pharmacol Exp Ther 236:819–832.Google Scholar
  8. 8.
    Terenghi G, McGregor GP, Bhuttacharji S, Wharton J, Bloom SR, Polak JM (1983) Vagal origin of substance Pcontaining nerves in the guinea-pig lung. Neurosci Lett 36:229–236.PubMedCrossRefGoogle Scholar
  9. 9.
    Barnes PJ (1984) The third nervous system in the lung: physiology and clinical perspectives. Thorax 139:561-567.CrossRefGoogle Scholar
  10. 10.
    Coburn RF, Tomita T (1973) Evidence for nonadrenergic inhibitory nerves in the guinea-pig trachealis muscle. Am J Physiol 224:1072–1080.PubMedGoogle Scholar
  11. 11.
    Nilsson G, Dahlberg K, Brodin E, Sundler F, Strandberg K (1977) Distribution and constrictor effect of substance P in guinea-pig tracheobronchial tissue. In: von Euler US, Pernow B (eds) Substance P. New York: Raven Press.Google Scholar
  12. 12.
    Stephenson JA, Burcher E, Summers RJ (1986) Autoradiographic demonstration of endothelium-dependent binding of 125I-Bolton-Hunter substance P to dog carotid artery. Eur J Pharmaco1 124:377–378.CrossRefGoogle Scholar

Copyright information

© Springer-Verlag New York Inc. 1987

Authors and Affiliations

  • D. J. Watkins
    • 1
  • E. Burcher
    • 1
  1. 1.Division of Biological and Health SciencesDeakin UniversityAustralia

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