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Tyr8-Substance P Binding Study on a Plasma Membrane from Dog Ileum Smooth Muscle

  • M. J. Muller
  • H. Sato
  • E. E. Daniel
Conference paper

Abstract

Binding of 125I-Tyr8-substance P (125I-Tyr8-SP) to a plasma membrane enriched fraction (PM) from the circular muscle of canine small intestine was studied. The binding was fast, reversible and saturable with a KD and Bmax of 0.5 ± 0.06 nm and 742 ± 173 fmol/mg protein, respectively. 125Ieledoisin did not bind to PM. These results together with competition studies suggest that PH possesses SP-P receptors but not SP-E receptors.

Keywords

Circular Muscle Tachykinin Receptor Plasma Membrane Enzyme Circular Muscle Strip Canine Small Intestine 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag New York Inc. 1987

Authors and Affiliations

  • M. J. Muller
    • 1
  • H. Sato
    • 1
  • E. E. Daniel
    • 1
  1. 1.Department of Neurosciences, Faculty of Health SciencesMcMaster UniversityHamiltonCanada

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