Effects of Substance P on the Binding of Ligands to Nicotinic Acetylcholine Receptors on Torpedo Electroplaque Membranes
Substance P has been shown to noncompetitively inhibit nicotinic acetylcholine receptor (nAChR) responses in a number of experimental systems. These include among others, spinal Renshaw cells [1,2], adrenal chromaffin cells [3,4], a neuronal cell line (PCl2) [5,6], and a muscle-like cell line (BC3HI) . The effect of substance P has been shown to be specific for the nAChR; substance P fails to alter excitation responses by glutamate, aspartate, or muscarinic agoinsts [1,2], and fails to block secretion induced by depolarization by high potassium or veratridine [3,4]. It has recently been shown that the site through which substance P exerts this effect is probably different from sites previously described for the peptide .
KeywordsNicotinic Acetylcholine Receptor Neuronal Cell Line Adrenal Chromaffin Cell Renshaw Cell Agonist Binding Site
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