Endothelial-Dependent Binding of [125I]-Bolton-Hunter Substance P in Blood Vessels

  • J. A. Stephenson
  • R. J. Summers
  • E. Burcher
Conference paper


Substance P is one of the most potent vasodilators known [1] and the vasodilatation in some dog, cat and rabbit arteries is endothelium-dependent [2]. Endothelium-dependent relaxation by Substance P has since been shown in rings of dog common carotid arteries [3], pig and dog coronary arteries [4] and in vivo in dog femoral artery [5]. This vasodilatation may be mediated by release of endothelium-derived relaxing factor (EDRF) [2]. In the study described here, we have used the techniques of in vitro radioligand binding and autoradiography [6] to detect the localization of [125I] Bolton-Hunter substance P (BHSP) to sections of blood vessels with the endothelium intact and to similar preparations with the endothelium removed.


Methylene Blue Intact Endothelium Autoradiographic Localization Bovine Aorta Light Microscopic Autoradiography 
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Copyright information

© Springer-Verlag New York Inc. 1987

Authors and Affiliations

  • J. A. Stephenson
    • 1
  • R. J. Summers
    • 1
  • E. Burcher
    • 2
  1. 1.Department of PharmacologyUniversity of MelbourneVictoriaUSA
  2. 2.Department of Biological and Health SciencesDeakin UniversityVictoriaAustralia

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