Biosynthesis of Substance P and Neurokinin A in the Enteric Nervous System

  • C. F. Deacon
  • J. M. Conlon
Conference paper


Immunocytochemical studies [1–3] have demonstrated that the guinea pig small intestine is supplied with a complex network of substance P-containing neurons. Extrinsic neurons project to the submucosal blood vessels and to the submucous ganglia. Intrinsic neurons in the submucous ganglia supply the villi and projections from the myenteric plexus supply most regions of the gut. Substance P-immunoreactive cell bodies are most numerous in the myenteric plexus. Recent studies have shown that extracts of guinea pig ileum contain neurokinin A and neuropeptide K [4] and these peptides have been identified in the same population of spinal ganglion cells and capsaicin-sensitive nerve fibres in the guinea pig ureter, inferior mesenteric. ganglion, heart and lung [5]. Biosynthesis in vitro of substance P [6] and neurokinin A [7] has previously been demonstrated in rat dorsal root ganglia and experiments in vivo have shown de novo synthesis of substance P in the rat corpus striatum and its transport to the substantia nigra [8]. This study extends this previous work to demonstrate in vitro biosynthesis of substance P, neurokinin A and neuropeptide K in enteric neurons of the myenteric plexus of the guinea pig ileum.


Myenteric Plexus Capsaicin Sensitive Sensory Neuron Longitudinal Muscle Strip Submucous Ganglion Spinal Ganglion Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Costa M, Furness JB, Llewellyn-Smith IJ, Cuello AC (1981) Projections of substance P-containing neurons within the guinea pig small intestine. Neuroscience 6:411–424PubMedCrossRefGoogle Scholar
  2. 2.
    Schultzberg M, Hökfelt T, Nilsson G, Terenius L, Rehfeld JF, Brown M, Elde R, Goldstein M, Said S (1980) Distribution of peptide- and catecholamine-containing neurons in the gastrointestinal tract of rat and guinea pig: Immunohistochemical studies with antisera to substance P, vasoactive intestinal polypeptide, enkephalins, somatostatin, gastrin/cholecystokinin, neurotensin anddopamine β-hydroxylase.Neuroscience 5:689–744PubMedCrossRefGoogle Scholar
  3. 3.
    Jessen KR, Saffrey MJ, Van Noorden S, Bloom SR, Polak JM, Burnstock G (1980) Immunohistochemical studies on the enteric nervous system in tissue culture and in situ:localization of vasoactive intestinal polypeptide (VIP), substance P and enkephalin immunoreactive nerves in the guinea-pig gut. Neuroscience 5:1717–1735PubMedCrossRefGoogle Scholar
  4. 4.
    Deacon CF, Agoston DV, Nau R, Conlon JM (1986) Conversion of neuropeptide K to neurokinin A and vesicular colocalisation of neurokinin A and substance P in neurons of the guinea pig small intestine. Submitted for publicationGoogle Scholar
  5. 5.
    Hua X-Y, Theodorsson-Norheim E, Brodin EM, Lundberg JM, Hökfelt T (1985) Multiple tachykinins (neurokinin A, neuropeptide K and substance p) in capsaicin sensitive sensory neurons in the guinea pig. Regul Pept 13:1–19PubMedCrossRefGoogle Scholar
  6. 6.
    Harmar A, Schofield JG, Keen P (1981) Substance P biosynthesis in dorsal root ganglia: an immunochemical study of [35S]methionine and [3H]proline incorporation in vitro. Neuroscience 6:1917–1922CrossRefGoogle Scholar
  7. 7.
    Hamar AJ, Keen P (1984) Rat sensory ganglia incorporate radiolabelled amino acids into substance K (neurokinin α) in vitro. Neurosci Lett 51: 387–391CrossRefGoogle Scholar
  8. 8.
    Sperk G, Singer EA (1982) In vivo synthesis of substance P in the corpus striatum of the rat and its transport to the substantia nigra. Brain Res 238:127–135PubMedCrossRefGoogle Scholar
  9. 9.
    Paton WDM, Zar MA (1968) The origin of acetylcholine released from guinea pig intestine and longitudinal muscle strips. J.Physiol (Lond.) 194:13–33Google Scholar
  10. 10.
    Conlon JM, Deacon CF, Richter G, Schmidt WE, Stöckmann F, Creutzfeldt W (1986) Measurement and partial characterization of the multiple forms of neurokinin A-like immunoreactivity in carcinoid tumours. Regul Pept 13:183–186PubMedCrossRefGoogle Scholar
  11. 11.
    Lee CM, Emson PC, Iversen LL (1980) The development and application of a novel N-terminal directed substance P antiserum. Life Sci 27:535–543PubMedCrossRefGoogle Scholar
  12. 12.
    Tatemoto K, Lundberg JM, Jörnvall H, Mutt V (1985) Neuropeptide K: Isolation, structure and biological activities of a novel brain tachykinin. Biochem Biophys Res Comm 124:751–738Google Scholar

Copyright information

© Springer-Verlag New York Inc. 1987

Authors and Affiliations

  • C. F. Deacon
    • 1
  • J. M. Conlon
    • 1
  1. 1.Clinical Research Group for Gastrointestinal Endocrinology of the Max-Planck-SocietyUniversity of GöttingenGöttingenGermany

Personalised recommendations