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Gene Transfer into the Nervous System

  • Xandra O. Breakefield
  • Alfred I. Geller
Part of the Molecular Neurobiology book series (MN)

Abstract

Several techniques have been developed that allow the introduction of recombinant DNA molecules into the cells of the nervous system. These methods include DNA uptake (transfection), microinjection of DNA, and infection with viral vectors. Gene transfer can be used for two general purposes: (1) To evaluate the regulation and function of a cloned gene following its modification and introduction into different cell types. Such studies include the definition of the regulatory elements that control levels of gene expression, alternate modes of RNA splicing, post-translational processing of peptides, sorting of proteins to their appropriate cellular locations, and biological activities of proteins. (2) To modify cells of the nervous system in culture and in vivo.

Index Entries

Gene transfer retrovirus herpes transgenic mice viral vectors nervous system 

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Copyright information

© The Humana Press Inc. 1988

Authors and Affiliations

  • Xandra O. Breakefield
    • 1
    • 2
    • 3
  • Alfred I. Geller
    • 1
    • 2
    • 3
  1. 1.Molecular NeurogeneticsEunice Kennedy Shriver CenterWalthamUSA
  2. 2.Laboratory of NeurogeneticsMassachusetts General HospitalBostonUSA
  3. 3.Neuroscience Program (Neurology)Harvard Medical SchoolBostonUSA

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