Comparison of Interleukin-1 with Endogenous B-Cell Factors for Effects on Lymphoma Cell Growth After EBV Conversion
Epstein-Barr Virus (EBV) transforms human B lymphocytes so that they grow as continuous cell lines. B lymphocyte lines transformed in vitro by EBV (LCL) and B lymphocyte lines derived from EBV positive and EBV negative Burkitt’s lymphomas (BL) release a 15–25 K growth enhancing supernatant (GE-S) factor(s) which functions similarly to macrophage derived Interleukin-1 (IL-1). GES and purified IL-1 were compared and both enhanced growth of LCL and BL similarly. Preliminary data show growth of Ramos, an EBV negative BL, is not changed by GE-S or 1L-1, whereas growth of EBV-converted Ramos sublines is enhanced by the addition of either. These initial findings suggest that EBV infection induces the development of growth factor sensitivity in lymphoma cells.
KeywordsBurkitis Lymphoma Lymphoblastoid Cell Line Lymphocyte Line Virus Transformed Cell Size Exclusion HPLC
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- 4.Blazar, B.A., Rodrick, N.L., Sutton, L.M, and Strom, M. Manuscript submitted.Google Scholar