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Detection of Epstein-Barr Virus DNA in Nasopharyngeal Biopsies

  • H.-Y. Zhang
  • G. Qu
  • Z.-W. Deng
  • T.-H. Yao
  • R. Glaser
Part of the Experimental Biology and Medicine book series (EBAM, volume 15)

Abstract

Epstein-Barr Virus (EBV) is closely associated with undifferentiated nasopharyngeal carcinoma (NPC) and Burkitt’s lymphoma (BL) where the virus genome is stably maintained and only partially expressed, e.g., the nuclear antigen (EBNA). The possibility of integration of EBV DNA into cellular DNA in latently infected nasopharyngeal epithelial cells over years of active cell replication is supported by the finding that viral DNA is localized on both chromatids of one homologue of chromosome 1 in Namalwa (a BL cell line) and on both chromatids of one homologue of chromosome 4 in IB4 (a cell line with transformed growth properties) (1). The results of restriction mapping of Raji cellular DNA obtained after separation on CsCl gradients suggest that viral DNA is integrated into cellular DNA at the region of 52032 end of the BamHl W fragment (2). It is not understood how EBV is maintained in NPC tumor cells.

Keywords

Nasopharyngeal Epithelial Cell Undifferentiated Nasopharyngeal Carcinoma Nasopharyngeal Biopsy CsC1 Gradient EcoRI Cleavage 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    A. Henderson, S. Ripley, M. Miller, et al., Proc. Natl. Acad. Sci. USA, 80, 1987 (1983).PubMedCrossRefGoogle Scholar
  2. 2.
    M. Anvret, A. Karlsson and G. Bjursell, Nucleic Acids Research, 12, 1149 (1984).PubMedCrossRefGoogle Scholar

Copyright information

© The Humana Press Inc. 1987

Authors and Affiliations

  • H.-Y. Zhang
    • 1
  • G. Qu
    • 3
  • Z.-W. Deng
    • 3
  • T.-H. Yao
    • 3
  • R. Glaser
    • 1
    • 2
  1. 1.College of MedicineThe Ohio State UniversityColumbusUSA
  2. 2.Comprehensive Cancer CenterThe Ohio State UniversityColumbusUSA
  3. 3.Academia SinicaShanghai Institute of Cell BiologyShanghaiP.R.C.

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