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Effects of S-adenosylhomocysteine and Analogs on Epstein-Barr Virus (EBV)-Induced Transformation, EBV DNA Methylation and Gene Expression

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Epstein-Barr Virus and Human Disease

Part of the book series: Experimental Biology and Medicine ((EBAM,volume 15))

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Abstract

EBV DNA becomes increasingly methylated with time following cell transformation (1) while hypomethylation has been associated with expression of viral genes (2). S-adenosylhomocysteine (SAH), a product of the S-adenosylmethionine transmethylation reaction, acts as a competitive inhibitor of DNA methyltransferases (3). In the present study SAH and analogs, sinefungin (SF) and 5′-deoxy-5′-S-isobutyladenosine (SIBA), as well as 5-azacytidine, which inhibits methylation of nascent DNA (4), were utilized in studies to determine the role of DNA methylation in the regulation of Epstein-Barr virus (EBV) functions such as transformation of human peripheral blood lymphocytes (PBL) and gene expression in lymphoblastoid cell lines. SF and SIBA, but not SAH, inhibited EBV-induced transformation of human PBLs. Indirect immunofluorescence and flow cytometric analyses revealed a significant increase in viral capsid antigen expression in lymphoblastoid cell lines following exposure to the analogs SF and SIBA, but not SAH.

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References

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Author information

Authors and Affiliations

  1. Department of Microbiology and Immunology, Temple University School of Medicine, Philadelphia, PA, 19140, USA

    Gerald Fronko, Earl Henderson & Walter Long

  2. Flow Cytometry Laboratory, Albert Einstein Medical Center, Philadelphia, PA, 19140, USA

    Brian Wu

Authors
  1. Gerald Fronko
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  2. Earl Henderson
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  3. Brian Wu
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  4. Walter Long
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Editor information

Editors and Affiliations

  1. National Cancer Institute, Bethesda, Maryland, USA

    P. H. Levine  & D. V. Ablashi  & 

  2. Showa University Research Institute, St. Petersburg, Florida, USA

    M. Nonoyama

  3. Georgetown University Medical Center, Washington, D C, USA

    G. R. Pearson

  4. The Ohio State University, Columbus, Ohio, USA

    R. Glaser

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© 1987 The Humana Press Inc.

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Fronko, G., Henderson, E., Wu, B., Long, W. (1987). Effects of S-adenosylhomocysteine and Analogs on Epstein-Barr Virus (EBV)-Induced Transformation, EBV DNA Methylation and Gene Expression. In: Levine, P.H., Ablashi, D.V., Nonoyama, M., Pearson, G.R., Glaser, R. (eds) Epstein-Barr Virus and Human Disease. Experimental Biology and Medicine, vol 15. Humana Press. https://doi.org/10.1007/978-1-4612-4590-2_32

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  • DOI: https://doi.org/10.1007/978-1-4612-4590-2_32

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-4612-8940-1

  • Online ISBN: 978-1-4612-4590-2

  • eBook Packages: Springer Book Archive

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