Autoradiographic Studies of Serotonin Receptors
Our perception of the chemical organization of the brain has been dramatically modified in the last 20 years by the results of the application of techniques such as histofluorescence, immuno-histochemistry, and autoradiography to the study of neurotransmitters. Serotonin has not been an exception but, rather, a forerunner. Serotonergic pathways have been studied using fluorescence microscopy, autoradiography, and immunohistochemistry. In 1964 Dahlström and Fuxe described the distribution of the serotoninergic cell bodies using the fluorescence technique of Falck et al. (1962). Autoradiography has been extensively used by Descarries and collaborators (Beaudet and Descarries, 1978; Parent et al., 1981) to trace 5-HT pathways. Finally, the development of antibodies has opened a new era in the research of 5-HT neurons in the brain, and allowed detailed investigation at the light- and electron-microscopic levels (Steinbush, 1981). More recently, these anatomical studies have been extended to the localization of receptor sites for this neurotransmitter.
KeywordsDopamine Serotonin Catecholamine Fluoxetine Lidum
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