Abstract
Using an antiserum to lys-vasopressin (Incstar), a small population of cells is stained which is different from that reported previously in insects. This study was done using an indirect immunofluorescent method (Davis, 1987) applied to the CNS of Lymantria dispar (gypsy moth) and Man-duca sexta. The antiserum was used at working dilutions 1:2000. Preabsorbtion of the working solution with synthetic arg- and lys-vasopressin (1 uM/ml) completely abolished the staining. The vasopressin-like immunoreceptive (VLI) cells were not stained by an antiserum specific to arg-vasopressin (Peninsula Laboratories) nor by an antiserum to oxytocin (Chemicon). These results suggest that the VLI material has epitopes common to lys- and arg-vasopressin.
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References
Davis, N. T. (1987) Neurosecretory neurons and their projection into the serotonin neurohemal system of the cockroach Periplaneta americana (L.), and identification of motor neurons associated with this system. J. Comp. Neurol. 259: 604–621.
Maddrell, S.H.P. (1986) Hormonal control of diuresis in insects. In Insect Neurochemistry and Neurophysiology (eds. A.B. Borkovec and D.B. Gelman). Humana, Clifton NJ.
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© 1990 The Humana Press Inc.
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Davis, N.T., Lehman, H. (1990). A Vasopressin-Like Neurohemal System in Lymantria Dispar and Manduca Sexta . In: Borkovec, A.B., Masler, E.P. (eds) Insect Neurochemistry and Neurophysiology · 1989 ·. Experimental and Clinical Neuroscience. Humana Press. https://doi.org/10.1007/978-1-4612-4512-4_65
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DOI: https://doi.org/10.1007/978-1-4612-4512-4_65
Publisher Name: Humana Press
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