Developmental Profiles for the Regulation of Ecdysteroid Production by the Prothoracic Glands of Female Gypsy Moth, Lymantria Dispar, Pupae
Activation of prothoracic glands (PGs) by their primary effector, prothoracicotropic hormone (PTTH), results in the production (synthesis and release) of ecdysteroids at precise times during larval and pupal life (Bollenbacher, 1988; Bollenbacher and Granger, 1985). An in vitro method has been developed by Bollenbacher et al. (1979, 1983) to quantify the PTTH-activation of PGs from larval or pupal Manduca sexta. This in vitro approach was used to quantify kinetic and developmental changes in the activity of PGs from female L. dispar pupae before and after PTTH stimulation. This information was used to establish an in vitro assay for PTTH using PGs from female L. dispar pupae.
Unable to display preview. Download preview PDF.
- Bollenbacher W. E. and Granger N. A. (1985) Endocrinology of the prothoracicotropic hormone. In Comprehensive Insect Physiology, Biochemistry and Pharmacology (Ed. by Kerkut G. A. and Gilbert L. I. ), Vol. 8, pp. 109–151, Pergamon Press, New York.Google Scholar
- Bollenbacher W. E., Agui N., Granger N. A. and Gilbert L. I. (1979) In vitro activation of insect prothoracic glands by the prothoracicotropic hormone. Proc. Natl. Acad. Sci. USA 76, 5148–5152.Google Scholar
- Kelly T. J., Masler E. P., Thyagaraja B. S., Bell R. A. and Borkovec A. B. (1986) cc. In Insect Neurochemistry and Neurophysiology (Ed. Borkovec A. B. and Gelman D. B.), pp. 327–330, Humana Press, Clifton, New Jersey.Google Scholar