Developmental Profiles for the Regulation of Ecdysteroid Production by the Prothoracic Glands of Female Gypsy Moth, Lymantria Dispar, Pupae
Activation of prothoracic glands (PGs) by their primary effector, prothoracicotropic hormone (PTTH), results in the production (synthesis and release) of ecdysteroids at precise times during larval and pupal life (Bollenbacher, 1988; Bollenbacher and Granger, 1985). An in vitro method has been developed by Bollenbacher et al. (1979, 1983) to quantify the PTTH-activation of PGs from larval or pupal Manduca sexta. This in vitro approach was used to quantify kinetic and developmental changes in the activity of PGs from female L. dispar pupae before and after PTTH stimulation. This information was used to establish an in vitro assay for PTTH using PGs from female L. dispar pupae.
KeywordsGypsy Moth Female Pupa Primary Effector Tobacco Hornworm Prothoracic Gland
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