Stimulation of de novo Pheromone Biosynthesis by In Vitro Pheromone Glands of Heliothis SPP

  • A. Rafaeli
  • V. Soroker
  • J. A. Klun
  • A. K. Raina
Part of the Experimental and Clinical Neuroscience book series (ECN)

Abstract

We have recently demonstrated (Soroker & Rafaeli, 1989) that isolated in vitro pheromone glands of Heliothis armigera incorporate radioactivity from 14C sodium acetate and that this total incorporation is stimulated by a pheromonotropic factor present in brain-complex extracts. This stimulation was found to be mediated by cyclic-AMP (Rafaeli & Soroker, 1989b). Subsequent TLC and HPLC separations of the radioactive products extracted by hexane revealed co-elution with Z (11)-hexadecenal (Z-11-16:ald). Furthermore, capillary GC analysis of the hexane soluble products from such incubations, in the absence of 14C sodium acetate, revealed a stimulation of the concentration of Z-11-16:ald by brain extracts. However, levels of pheromone attained were found to be lower than those attained by live virgin females (Rafaeli & Soroker, 1989a).

Keywords

HPLC Hexane Hexadecenal Hexadecenyl 

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References

  1. Rafaeli A. and Soroker, V. (1989a) Effect of diel-rhythm and brain hormone on pheromone production in two Lepidopteran species. J. Chem. Ecol. 15, 447–455.CrossRefGoogle Scholar
  2. Rafaeli A. and Soroker, V.(1989b) cAMP mediation of the hormonal stimulation of 14C-acetate incorporation by Heliothis armigera pheromone glands in vitro. Mol. & Cell. Endocr. (in press).Google Scholar
  3. Soroker, V. and Rafaeli, A. (1989) In vitro hormonal stimulation of acetate incorporation by Heliothis armigera pheromone glands. Insect Biochem. 67, 1–6.Google Scholar
  4. Tang J.D., Charlton, R.E., Carde, R.T. and Yin, C.-M. (1987) Effect of allatectomy and ventral nerve cord transection on calling, pheromone emission and pheromone production in Lymantria dispar. J. Insect Physiol. 33, 469–476.CrossRefGoogle Scholar

Copyright information

© The Humana Press Inc. 1990

Authors and Affiliations

  • A. Rafaeli
    • 1
  • V. Soroker
    • 1
  • J. A. Klun
    • 2
  • A. K. Raina
    • 2
  1. 1.Department of Entomology, Faculty of AgricultureThe Hebrew UniversityRehovotIsrael
  2. 2.Insect Chemical Ecology Lab.USDABeltsvilleUSA

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