Stimulation of de novo Pheromone Biosynthesis by In Vitro Pheromone Glands of Heliothis SPP
We have recently demonstrated (Soroker & Rafaeli, 1989) that isolated in vitro pheromone glands of Heliothis armigera incorporate radioactivity from 14C sodium acetate and that this total incorporation is stimulated by a pheromonotropic factor present in brain-complex extracts. This stimulation was found to be mediated by cyclic-AMP (Rafaeli & Soroker, 1989b). Subsequent TLC and HPLC separations of the radioactive products extracted by hexane revealed co-elution with Z (11)-hexadecenal (Z-11-16:ald). Furthermore, capillary GC analysis of the hexane soluble products from such incubations, in the absence of 14C sodium acetate, revealed a stimulation of the concentration of Z-11-16:ald by brain extracts. However, levels of pheromone attained were found to be lower than those attained by live virgin females (Rafaeli & Soroker, 1989a).
KeywordsHPLC Hexane Hexadecenal Hexadecenyl
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