Binding of Synthetic Dipteran Neuropeptide Preparations to Head and Abdominal Proteins of 3-Day Old Face Flies

  • Dora K. Hayes
Part of the Experimental and Clinical Neuroscience book series (ECN)

Abstract

Receptors for hormones function in both time and space. In order to learn how best to use neuropeptides in insect population management programs, determination of when most receptor activity is found in the insect and the nature of that receptor are legitimate avenues of investigation. Keeley and Hayes (1987) state that “practical disruption of hormone-receptor interactions depends on the future development of . . . hormone analogs designed from information of the biologically active structures of specific hormones. The structures of biological interest are those formed by peptide chains when they bind and activate target cell receptors.” I here report on proteins which bind to a neuropeptide, but which have not been specifically identified as receptors. The neuropeptide chosen for study was a synthetic molecule with an amino acid sequence identical to that for an adipokinetic-type hormone isolated from the ring glands of tabanids by Jaffe et al. (1988). Because vertebrate receptors have been shown to change with time over a 24 h span (Scheving et al. 1989, for example), binding of proteins from extracts prepared at different times after lights on (HALO) was determined.

Keywords

Albumin Fluoride Electrophoresis Polyacrylamide Diethyl 

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References

  1. Hayes D. K., Jaffe H., Morgan N.O. and Redfern R.E. (1986) Binding of synthetic insect peptide analogs to components of insect membrane preparations. In Insect Neurochemistry and Neurophysiology (Ed. by Borkovec A. B. and Gelman D. B. ), pp. 247–250. The Humana Press, Clifton, N.J.Google Scholar
  2. Jaffe H., Raina A.K., Fraser B.A., Keim P., Rao K.R., Zhang Y.S., Lancaster J.L. and Hayes D.K. (1988) Isolation of two neuropeptides in the AKH/RPCH family from horseflies (Diptera). Biochem. Biophys. Res. Communs. 151, 656–663.Google Scholar
  3. Keeley L. L. and Hayes T. K. (1987) Speculations on biotechnology applications for insect neuroendocrine research. Insect Biochem. 17, 639–651.CrossRefGoogle Scholar
  4. Scheving L. A., Tsai T. H., Cornett L. E. Feuers R. J. and Scheving L. E. (1989). Circadian variation of epidermal growth factor receptor in mouse liver. Chronobiologia 16, 177–178.Google Scholar

Copyright information

© The Humana Press Inc. 1990

Authors and Affiliations

  • Dora K. Hayes
    • 1
  1. 1.Livestock Insects Lab., L.P.S.I.Agr. Res. Serv. U. S. Dept. Agr.BeltsvilleUSA

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