Anti EBNA-1 Protein Induced with TPA

  • L. T. Wen
  • A. Tanaka
  • M. Nonoyama
Chapter
Part of the Experimental Biology and Medicine book series (EBAM, volume 20)

Summary

Binding of the EBNA-1 to ori-P was studied by affinity column chromatography followed by immunoblotting with anti EBNA-1 serum. Treatment of Raj i cells with phorbol ester, which activates the EBV replicative life cycle, induced the production of two cellular competitors which upon competition uncoupled the binding of EBNA-1 to ori-P. Gel retardation assay, DNase I footprinting and methylation interference analysis showed that the two cellular competitors bound specifically to the 20x 30 bp tandem repeat clusters in the EBV ori-P.

Keywords

Cellulose Purine Phorbol 

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References

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Copyright information

© The Humana Press Inc. 1989

Authors and Affiliations

  • L. T. Wen
    • 1
  • A. Tanaka
    • 1
  • M. Nonoyama
    • 1
  1. 1.Department of VirologyShowa University Research Institute for Biomedicine in FloridaSt. PetersburgUSA

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