Quantification of Specific Nucleic Acids, Regulated RNA Processing, and Genomic Polymorphisms Using Reversed-Phase HPLC

  • A. Hayward-Lester
  • B. S. Chilton
  • P. A. Underhill
  • P. J. Oefner
  • P. A. Doris
Part of the Advanced Biomedical Technologies book series (ABT)


The ability to make accurate quantitative measurements of specific nucleic acid molecules is becoming increasingly significant in biomedical science. One important clinical application is the quantification of viral nucleic acids in patients’ serum and tissues to assess viral burden for determination of disease course and efficacy of treatment. In this case, accuracy and precision of measurement are of primary importance, as decisions concerning selection of medication and dose may depend entirely on perceived change in viral load in an otherwise asymptomatic patient. A second utility in basic biomedical research is the quantification of specific mRNA molecules in functionally characterized cell types. These measurements are useful because they provide a prediction of protein abundance in cell samples in which proteins cannot be quantified directly. These studies often require microdissection to separate the tissues of interest from neighboring tissues. Again, accurate quantification is important for the effective comparison of the expression of multiple genes in multiple tissues and at different laboratories, often under conditions of physiological or pharmacological manipulation.


Gene Quantification Nephron Segment Heteroduplex Molecule Mutant Template Principal Reaction Product 
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Copyright information

© Birkhäuser Boston 1998

Authors and Affiliations

  • A. Hayward-Lester
    • 1
  • B. S. Chilton
    • 1
  • P. A. Underhill
    • 2
  • P. J. Oefner
    • 2
  • P. A. Doris
    • 3
  1. 1.Department of Cell Biology and BiochemistryTexas Tech University School of MedicineLubbockUSA
  2. 2.Department of BiochemistryStanford UniversityStanfordUSA
  3. 3.Institute of Molecular MedicineUniversity of Texas Health Sciences CenterHoustonUSA

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