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Filoviridae: Marburg and Ebola Viruses

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Laboratory Diagnosis of Infectious Diseases Principles and Practice

Abstract

Disease: Marburg hemorrhagic fever, Ebola hemorrhagic fever.

Etiologic Agents: Marburg virus, Ebola-Sudan virus, Ebola-Zaire virus.

Source: Unknown.

Clinical Manifestations: Acute onset with headache, myalgia, and lethargy. Leads to gastrointestinal symptoms and rash. Serious cases involve severe hemorrhagic manifestations. Death follows blood loss and shock. Mortality can be near 90% in an outbreak.

Pathology: Virus is present in most organs, and lesions are found in many organs. Vascular collapse leading to shock may be due to endothelial damage.

Laboratory Diagnosis: Diagnosis is made by virus isolation from blood and other tissues or by a fourfold rise in indirect fluorescence antibody titer. Caution must be used when handling specimens as viruses are extremely virulent.

Epidemiology: Viruses are indigenous to Africa.

Treatment: None proven.

Prevention and Control: No vaccine is available. Control is by good barrier nursing techniques and proper use of syringes and needles.

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Literature Cited

  • Baron, R. C., J. B. McCormick, and O. A. Zubeir. 1983. Ebola virus disease in southern Sudan: hospital dissemination and intrafamilial spread. Bull. W.H.O. 61: 997–1003.

    PubMed  CAS  Google Scholar 

  • Bowen, E. T. W., D. I. H. Simpson, W. F. Bright, I. Zlotnik, and D. M. R. Howard. 1969. Vervet monkey disease: studies on some physical and chemical properties of the causative agent. Br. J. Exp. Pathol. 50: 400–407.

    PubMed  CAS  Google Scholar 

  • Bowen, E. T. W., G. Lloyd, W. J. Harris, G. S. Piatt, A. Baskerville, and E. E. Vella. 1977. Viral hemorrhagic fever in southern Sudan and northern Zaire, preliminary studies on the aetiological agent. Lancet 1: 571–573.

    Article  PubMed  CAS  Google Scholar 

  • Buchmeier, M. J., R. U. DeFries, J. B. McCormick, and M. P. Kiley. 1983. Comparative analysis of the structural polypeptides of Ebola viruses from Sudan and Zaire. J. Infect. Dis. 147: 276–281.

    Article  PubMed  CAS  Google Scholar 

  • Centers for Disease Control. 1980. Marburg virus disease-Kenya. Morbid. Mortal. Weekly Rep. 29: 145–146.

    Google Scholar 

  • Centers for Disease Control. 1983. Viral hemorrhagic fever: initial management of suspected and confirmed cases. Morbid. Mortal. Weekly Rep. 32(suppl): 275–395.

    Google Scholar 

  • Cox, N. J., J. B. McCormick, K. M. Johnson, and M. P. Kiley. 1983. Evidence for two subtypes of Ebola virus based on oligonucleotide mapping of RNA. J. Infect. Dis. 147: 272–275.

    Article  PubMed  CAS  Google Scholar 

  • Dietrich, M., H. H. Schumacher, D. Peters, and J. Knobloch. 1978. Human pathology of Ebola (Maridi) virus infection in the Sudan, p. 37–42. InPattyn S. R. (ed. ), Ebola virus haemorrhagic fever.

    Google Scholar 

  • Elsevier/North Holland, Amsterdam, New York. Elliott, L. H., J. B.McCormick, and K. M. Johnson. 1982. Inactivation of Lassa, Marburg, and Ebola viruses by gamma irradiation. J. Clin. Microbiol. 16: 704–708.

    Google Scholar 

  • Elliott, L. H., M. P. Kiley, and J. B. McCormick. 1985. Descriptive analysis of Ebola virus proteins. Virology 147: 169–176.

    Article  PubMed  CAS  Google Scholar 

  • Fisher-Hoch, S. P., G. S. Piatt, G. H. Neild, T. Southee, A. Baskerville, R. T. Raymond, G. Lloyd, and D. I. H. Simpson. 1985. Pathophysiology of shock and hemorrhage in a fulminating viral infection (Ebola). J. Infect. Dis. 152: 887–894.

    Article  PubMed  CAS  Google Scholar 

  • Gear, J. S. S., G. A. Cassell, A. J. Gear, B. Trappier, L. Clausen, A. M. Meyers, M. C. Kew, T. H. Bothwell, R. M. Sher, G. B. Miller, J. Schneider, H. J. Koornhoff, E. D. Gomperts, M. Isaacson, and J. H. S. Gear. 1975. Outbreak of Marburg virus disease in Johannesburg. Br. Med. J. 4: 489–493.

    Article  PubMed  CAS  Google Scholar 

  • Johnson, B. K., D. Ocheng, S. Oogo, L. G. Gitau, C. Wambui, A. Gichogo, D. Libondo, P. M. Tukei, and E. J. Johnson. 1986. Seasonal variation in antibodies against Ebola virus in Kenyan fever patients. Lancet 1:1160. Johnson, K. M., P. A. Webb, J. V. Lange, and F. A. Murphy. 1977. Isolation and partial characterization of a new virus causing acute haemorrhagic fever in Zaire. Lancet 1: 569–571.

    Google Scholar 

  • Johnson, K. M., L. H. Elliott, and D. L. Heymann. 1981. Preparation of polyvalent viral immunofluorescent intracellular antigens and use in human serosurveys. J. Clin. Microbiol. 14: 527–529.

    PubMed  CAS  Google Scholar 

  • Kiley, M. P., E. T. W. Bowen, G. A. Eddy, M. Isaacson, K. M. Johnson, J. B. McCormick, F. A. Murphy, S. R. Pattyn, D. Peters, O. W. Prozesky, R. L. Regnery, D. I. H. Simpson, W. Slenczka, P. Sureau, G. van der Groen, P. A. Webb, and H. Wulff. 1982. Filoviridae: a taxonomic home for Marburg and Ebola viruses? Inter-virology 1824: 32.

    Google Scholar 

  • Kiley, M. P., E. T. W. Bowen, G. A. Eddy, M. Isaacson, K. M. Johnson, J. B. McCormick, F. A. Murphy, S. R. Pattyn, D. Peters, O. W. Prozesky, R. L. Regnery, D. I. H. Simpson, W. Slenczka, P. Sureau, G. van der Groen, P. A. Webb, and H. Wulff. 1982. Filoviridae: a taxonomic home for Marburg and Ebola viruses? Inter-virology 1824: 32.

    Google Scholar 

  • Murphy, F. A. 1978. Pathology of Ebola virus infection, p. 43–60. In: Pattyn S. R. (ed.), Ebola virus haemorrhagic fever, Elsevier/North Holland Biomedical Press, Amsterdam.

    Google Scholar 

  • Murphy, F. A., G. van der Groen, S. G. Whitfield, and J. V. Lange. 1978. Ebola and Marburg virus morphology and taxonomy, p. 61–84. InPattyn S. R. (ed.), Ebola virus haemorrhagic fever, Elsevier/North Holland Biomedical Press, Amsterdam.

    Google Scholar 

  • Pattyn, S., G. van der Groen, W. Jacob, P. Piot, and G. Courteille. 1977. Isolation of Marburg-like virus from a case of haemorrhagic fever in Zaire. Lancet 1: 573–574.

    Article  PubMed  CAS  Google Scholar 

  • Peters, D., G. Muller, and W. Slenczka. 1971. Morphology, development and classification of the Marburg virus, p. 68–83. InG. A. Martini and R. Siegert (eds.), Marburg virus disease. Springer-Verlag, New York.

    Google Scholar 

  • Regnery, R. L., K. M. Johnson, and M. P. Kiley. 1980. Virion nucleic acid of Ebola virus. J. Virol. 36:465–469.

    PubMed  CAS  Google Scholar 

  • Regnery, R. L., K. M. Johnson, and M. P. Kiley. 1981. Marburg and Ebola viruses: possible members of a new group of negative strand viruses, p. 971–977. InD. H. L. Bishop and R. W. Compans (eds.), The replication of negative strand viruses. Elsevier/North Holland, Inc., New York.

    Google Scholar 

  • Richardson, J. H., and W. E. Barkley (eds.). 1984. Bio-safety in microbiological and biomedical laboratories: US Department of Health and Human Services (HHS publication no. (CDQ86–8395).

    Google Scholar 

  • Richman, D. D., P. H. Cleveland, J. B. McCormick, and K. M. Johnson. 1983. Antigenic analysis of strains of Ebola virus: identification of two Ebola virus serotypes. J. Infect. Dis. 147: 268–271.

    Article  PubMed  CAS  Google Scholar 

  • Saluzzo, J. F., J. P. Gonzalez, A. J. Georges, and K. M. Johnson. 1981. Antibodies against the Marburg virus among human populations in the southeastern Central African Republic. C. R. Acad. Sci. (Paris) 292: 29–31.

    CAS  Google Scholar 

  • Sanchez, A., and M. P. Kiley. 1987. Identification and analysis of Ebola virus messenger RNA. Virology 157: 414–420.

    Article  PubMed  CAS  Google Scholar 

  • Siegert, R., H. L. Shu, W. Slenczka, D. Peters, and G. Muller. 1967. Zur atiologie einer unbekannten, von affen ausgegangenen menschlichen infektionskrankheit. Dt. Med. Wschr. 92: 2341–2343.

    Article  CAS  Google Scholar 

  • Simpson, D. I. H. 1977. Marburg and Ebola virus infections: a guide for their diagnosis, management, and control. WHO offset publication no. 36, World Health Organization, Geneva.

    Google Scholar 

  • Smith, C. E. G., D. I. H. Simpson, E. T. W. Bowen, and I. Zlotnik. 1967. Fatal human disease from vervet monkeys. Lancet 2: 1119–1121.

    Article  PubMed  CAS  Google Scholar 

  • Stojkovic, Lj., M. Bordjoski, A. Gligic, and Z. Stefanovic. 1971. Two cases of Cercopithicus monkeys-associated haemorrhagic fever, p. 24–33. InG. A. Martini and R. Siegert (eds.), Marburg virus disease. Springer-Verlag, New York.

    Google Scholar 

  • Teepe, xR. G., B. K. Johnson, D. Ocheng, A. Gichogo, A. Langatt, A. Ngindu, M. Kiley, K. M. Johnson, and J. B. McCormick. 1983. A probable case of Ebola virus haemorrhagic fever in Kenya. East Afr. Med. J. 60: 718–722.

    CAS  Google Scholar 

  • Van der Waals, F. J., K. L. Pomeroy, J. Goudsmit, D. M. Asher, and D. C. Gajdusek. 1986. Hemorrhagic fever virus infections in an isolated rainforest area of central Liberia. Limitations of the indirect immunofluorescence slide test for antibody screening in Africa. Trop. Geogr. Med. 38: 209–214.

    Google Scholar 

  • Webb, P. A., K. M. Johnson, H. Wulff, and J. V. Lange. 1978. Some observations on the properties of Ebola virus, p. 91–94. InS. R. Pattyn (ed.), Ebola virus haemorrhagic fever. Elsevier/North Holland Biomedical Press, Amsterdam.

    Google Scholar 

  • W.H.O. International Study Team Report. 1976. Ebola haemorrhagic fever in Sudan, 1976. Bull. W.H.O. 56: 247–270.

    Google Scholar 

  • W.H.O. International Study Team Report. 1976. Ebola haemorrhagic fever in Zaire, 1976. Bull. W.H.O. 56: 271–293.

    Google Scholar 

  • Wulff, H., W. Slenczka, and J. H. S. Gear. 1978. Early detection of antigen and estimation of virus yield in specimens from patients with Marburg virus disease. Bull. W.H.O. 56: 633–639.

    PubMed  CAS  Google Scholar 

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  1. Michael P. Kiley
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© 1988 Springer-Verlag New York Inc.

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Kiley, M.P. (1988). Filoviridae: Marburg and Ebola Viruses. In: Laboratory Diagnosis of Infectious Diseases Principles and Practice. Springer, New York, NY. https://doi.org/10.1007/978-1-4612-3900-0_30

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  • DOI: https://doi.org/10.1007/978-1-4612-3900-0_30

  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4612-8394-2

  • Online ISBN: 978-1-4612-3900-0

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