Abstract
The heat shock genes of Drosophila provide a very convenient system for the study of gene activation. These genes are activated in response to elevated temperature, metabolic inhibitors, and other stresses, apparently using a mechanism common to all cell types. The genes encoding the small heat shock proteins, hsp 22, hsp 23, hsp 26 and hsp 28 are also activated in specific Drosophila tissues at specific times according to a program of developmental regulation. (For a review of work on the organization and expression of Drosophila heat shock genes see Southgate et al., 1985.) We have chosen to focus our analysis on hsp 26, because of certain interesting features of the DNA in the 5’ regulatory region.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
References
Cartwright, I.L. and S.C.R. Elgin (1986). Nucleosomal instability and induction of new upstream protein-DNA associations accompany activation of four small heat shock protein genes in Drosophila melanogaster. Molec. Cell. Biol. 6: 779–791.
Church, G.M., and W. Gilbert (1984). Genomic sequencing. Proc. Natl. Acad. Sci. USA 81: 1991–1995.
Cohen, R.S., and M. Meselson (1985). Separate regulatory elements for the heat-inducible and ovarian expression of the Drosophila hsp 26 gene. Cell 43: 737–746.
Gilmour, D.S. and J.T. Lis (1986). RNA polymerase II interacts with the promoter region of the noninduced hsp 70 gene in Drosophila melanogaster cells. Mol. Cell. Biol. 6: 3984–3989.
Gilmour, D.S., and S.C.R. Elgin (1987). Localization of specific topoisomerase I interactions within the transcribed region of active heat shock genes by using the inhibitor camptothecin. Molec. Cell. Biol. 7: 141–148.
Gilmour, D.S., G. Pflugfelder, J.C. Wang, and J.T. Lis (1986). Topoisomerase I interacts with transcribed regions in Drosophila cells. Cell 44: 401–407.
Goto, T. and J.C. Wang (1985). Cloning of yeast TOP1, the gene encoding topoisomerase I, and the construction of mutants defective in both DNA topoisomerase I and DNA topoisomerase II. Proc. Natl. Acad. Sci. USA 82: 7178–7182.
Hsiang, Y.-H., R. Hertzberg, S. Hecht, and L.R. Lui (1985). Camptothecin induces protein-linked DNA breaks via mammalian DNA topoisomerase I. J. Biol. Chem. 260: 14873–14878.
Parker, C.S. and J. Topol (1984). A Drosophila RNA Polymerase II transcription factor specific for the heat shock gene binds to the regulatory site of an hsp 70 gene. Cell 37: 273–283.
Pauli, D., A. Spierer, and A. Tissieres (1986). Several hundred base pairs upstream of Drosophila hsp 23 and 26 genes are required for their heat induction in transformed flies. EMBO J. 5: 755–761.
Pelham, H.R.B. (1982). A regulatory upstream promoter element in the Drosophila hsp 70 heat shock gene. Cell 30: 517–528.
Seileck, S.B., S.C.R. Elgin, and I.L. Cartwright (1984). Supercoil-dependent features of DNA structure at Drosophila locus 67B1. J. Mol. Biol. 178: 17–33.
Siegfried, E., G.H. Thomas, U.M. Bond, and S.C.R. Elgin (1986). Characterization of a supercoil-dependent S1 sensitive site 5’ to the Drosophila melanogaster hsp 26 gene. Nuc. Acids. Res. 14: 9425–9444.
Simon, J.A. and J.T. Lis (1987). A germline transformation analysis reveals flexibility in the organization of heat shock consensus elements. Nuc. Acids Res. 15: in press.
Southgate, R., M.-E. Mirault, A. Ayme, and A. Tissieres (1985). Organization, sequences, and induction of heat shock genes, in “Changes in Eukaryotic Gene Expression in Response to Environmental Stress,” ed. by B.G. Atkinson and D.B. Waiden (Academic Press, N.Y.), pp. 3–30.
Uemura, T. and M. Yanagida (1984). Isolation of type I and II DNA topoisomerase mutants from fission yeast: single and double mutants show different phenotypes in cell growth and chromatin organization. EMBO J. 31: 1737–1744.
Wu, C. (1984) Activating protein factor binds in vitro to upstream control sequences in heat shock gene chromatin. Nature (London) 311: 81–84.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1988 Springer-Verlag New York, Inc.
About this chapter
Cite this chapter
Elgin, S.C.R., Cartwright, I.L., Gilmour, D.S., Siegfried, E., Thomas, G.H. (1988). Chromatin Structure and DNA Structure at the hsp 26 Locus of Drosophila . In: Wells, R.D., Harvey, S.C. (eds) Unusual DNA Structures. Springer, New York, NY. https://doi.org/10.1007/978-1-4612-3800-3_3
Download citation
DOI: https://doi.org/10.1007/978-1-4612-3800-3_3
Publisher Name: Springer, New York, NY
Print ISBN: 978-1-4612-8357-7
Online ISBN: 978-1-4612-3800-3
eBook Packages: Springer Book Archive