A large variety of DNA molecules, derived as restriction fragments from both prokaryotic (Ross et al., 1982; Stellwagen, 1983; Bossi and Smith, 1984; Zahn and Blattner, 1985) and eukaryotic (Simpson, 1979; Challberg and England, 1980; Israelewski, 1983; Schmidt, 1984; Kidane et al., 1984; Garrett and Carroll, 1986; Ray et al., 1986; Ryder et al., 1986) sources, display abnormal electrophoretic behavior. In particular, such molecules run more slowly in acrylamide gels than would be expected on the basis of their sizes (by sequence). An additional example of such electrophoretic behavior is displayed in figure 1. This example is of historical interest in that the HindII+III digest of SV40 was the first published restriction digest. The retardation of the F fragment was originally believed to be due to a chemical interaction of the fragment with the acrylamide gel. The example is also noteworthy in that the sequences giving rise to the anomalous behavior lie entirely within the coding sequences for the major capsid protein (VP1) gene.
KeywordsMigration Magnesium Recombination Agarose Electrophoresis
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