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Tissue Culture and Cryopreservation of Fetal Mammalian Endocrine Pancreas Intended for Transplantation

  • Chapter
Fetal Islet Transplantation

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Abstract

Transplantation of insulin-producing cells appears to be the ideal method to achieve perfect glycemic control in patients with insulin-dependent diabetes mellitus. Indeed, with the advent of new surgical techniques and improved immunosuppression therapy, whole or segmental adult pancreas transplantation has, during the last decade, become an increasingly successful treatment for these patients (1). However, it is already clear that the number of adult human pancreatic glands available is, and will remain, insufficient to meet the demands of patients requiring transplantation. It has therefore become necessary to explore the feasibility of transplanting other preparations of insulin-producing cells. One interesting source of β-cells in this context is the human fetal pancreas obtained from legal abortions. The exploration of this material as a source of transplantable tissue has been stimulated by the pioneering work of Brown and collaborators, which demonstrated that transplantation of syngeneic fetal rat pancreas reversed experimental diabetes mellitus in rats (2,3; see Introduction). The use of fetal pancreas transplantation in humans, however, introduces somewhat different problems from those encountered with transplants of the adult pancreas. The β-cell content of a single human fetal pancreas is not sufficient to immediately correct completely the hyperglycemia of an adult diabetic recipient, and thus successful outcome of the transplantation depends on significant expansion and differentiation of the implanted fetal β-cell mass.

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  1. Stellan Sandler
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  2. Arne Andersson
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  3. Ingemar Swenne
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  4. Leif Jansson
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  5. Olle Korsgren
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  6. Annika Schnell Landström
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  7. L. A. Håkan Borg
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  8. Birger Petersson
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  9. Carl-Gustav Groth
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  10. Claes Hellerström
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Editors and Affiliations

  1. Sansum Medical Research Foundation, Santa Barbara, CA, 93105, USA

    Charles M. Peterson M.D. (Director of Research), Lois Jovanovic-Peterson M.D. (Senior Scientist) & Bent Formby Ph.D., D.Sc. (Senior Biochemist) (Director of Research),  (Senior Scientist) &  (Senior Biochemist)

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Sandler, S. et al. (1988). Tissue Culture and Cryopreservation of Fetal Mammalian Endocrine Pancreas Intended for Transplantation. In: Peterson, C.M., Jovanovic-Peterson, L., Formby, B. (eds) Fetal Islet Transplantation. Springer, New York, NY. https://doi.org/10.1007/978-1-4612-3766-2_2

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  • DOI: https://doi.org/10.1007/978-1-4612-3766-2_2

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