Fermentation Development and Process Improvement

  • Mary Nallin Omstead
  • Louis Kaplan
  • Barry C. Buckland


Streptomyces avermitilis was originally isolated from soil as described in Chapter 2. The first fermentations of the culture used a standard screening approach: a loopful of slant growth was inoculated into a seed flask which was incubated and then transferred into two types of production flasks. Seed cultures were grown for 48 to 72 hours in baffled flasks containing 50 ml seed medium I (Burg et al. 1979). Production flasks were inoculated by transferring 2.0 ml of seed growth into 40 ml production medium A (Burg et al. 1979) and incubated for 4 days. The production cultures were then harvested and assayed for activity as indicated in Chapter 2.


Specific Growth Rate Shake Flask Production Medium Oxygen Transfer Oxygen Uptake Rate 
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© Springer-Verlag New York Inc. 1989

Authors and Affiliations

  • Mary Nallin Omstead
  • Louis Kaplan
  • Barry C. Buckland

There are no affiliations available

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