Molecular Approaches to Characterization of Mycoplasmal Adhesins

  • Shmuel Razin
  • David Yogev
Conference paper

Abstract

The mycoplasmas (class Mollicutes) constitute a very large and widespread group of parasitic prokaryotes, causing a variety of diseases in man, animals, plants and insects (Razin, 1978; Razin and Barile, 1985). They are distinguished from other prokaryotes by the total lack of a cell wall and by their minute dimensions. In fact, mycoplasmas can be considered as the smallest self-replicating organisms, closest to the concept of a “minimal cell” (Morowitz, 1984). The presence of only one membrane type, the plasma membrane, in mycoplasmas, combined with the ease of membrane isolation and manipulation of its lipid components, contributed to the establishment of this membrane as one of the most useful experimental models in membrane research (Razin, 1981). Recognition of the mycoplasma genome as the smallest among self-replicating organisms has a great appeal for those studying molecular biology, genetics and evolution, leading to significant progress in these aspects of recent mycoplasma research (Razin, 1985a; Woese, 1987). The great majority of mycoplasmas, which parasitize man and animals, colonize the epithelial linings of the respiratory and urogenital tracts.

Keywords

Codon Pneumonia Cysteine Serine Trypsin 

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References

  1. 1.
    Baseman, J.B., R.M. Cole, D.C. Krause, and D.K. Leith. 1982. Molecular basis for cytadsorption of Mycoplasma pneumoniae. J. Bacteriol. 151, 1514–1522.PubMedGoogle Scholar
  2. 2.
    Baseman, J.B., K.L. Daly, L.B. Trevino, and D.L. Drouillard. 1984a. Distinctions among pathogenic human mycoplasmas. isr.J.Med. Sci. 20, 866–869.Google Scholar
  3. 3.
    Baseman, J.B., D.L. Drouillard, D.K. Leith, and J.G. Tully. 1984b. Absence of Mycoplasma pneumoniae cytadsorption protein Pi in Mycoplasma genitalium and Mycoplasma gallisepticum. Infect.Immun. 43, 1103–1105.PubMedGoogle Scholar
  4. 4.
    Baseman, J.B., J. Morrison-Plummer, D. Drouillard, B. Puleo-Scheppke, V.V. Tryon, and S.C. Holt. 1987. Identification of a 32-kilodalton protein of Mycoplasma pneumoniae associated with hemadsorption. Isr.J.Med.Sci. 23, 474–479.PubMedGoogle Scholar
  5. 5.
    Biberfeld, G. 1985. Infection sequelae and autoimmune reactions in Mycoplasma pneumoniae infection, p. 293–311. In S. Razin and M.F. Barile (ed.), The Mycoplasmas, vol. 4: Mycoplasma Pathogenicity. Academic Press, Inc., Orlando, FL.Google Scholar
  6. 6.
    Bredt, W. 1979. Motility, p. 141–155. In M.F. Barile and S. Razin (ed.), The Mycoplasmas, vol. 1: Cell Biology. Academic Press, Inc. New York.Google Scholar
  7. 7.
    Bredt, W., B. Kleinmann, and E. Jacobs. 1987. Antibodies in the sera of Mycoplasma pneumoniae-infected patients against proteins of Mycoplasma genitalium and other mycoplasmas of man. Zbl.Bakt.Hyg. A 266, 32–42.Google Scholar
  8. 8.
    Cassell, G.H.,W.A. Clyde, Jr.,and J.K. Davis, 1985. Mycoplasmal respiratory infections, p. 69–106. In S. Razin and M.F. Barile (ed.), The Mycoplasmas, vol. 4: Mycoplasma Pathogenicity. Academic Press, Inc., Orlando, FL.Google Scholar
  9. 9.
    Clyde, W.A. Jr., and P.C. Hu. 1986. Antigenic determinants of the attachment proteins of M. pneumoniae shared by other pathogenic Mycoplasma species. Infect. Immun. 51, 690–692.PubMedGoogle Scholar
  10. 10.
    Dallo, S.F., C.J. Su, J.R. Horton, and J.B. Baseman. 1988. Identification of Pi gene domain containing epitope(s) mediating Mycoplasma pneumoniae cytadherence. J.Exp.Med. 167 (February issue).Google Scholar
  11. 11.
    Feldner, J., U. Gobel, and W. Bredt. 1982. Mycoplasma pneumoniae adhesin localized to tip structure by monoclonal antibody. Nature 298, 765–767.PubMedCrossRefGoogle Scholar
  12. 12.
    Fischer, M., and H. Kirchhoff. 1987. Interaction of Mycoplasma mobile 163K with erythrocytes. Zbl.Bakt.Hyg. A 266, 497–505.Google Scholar
  13. 13.
    Frydenberg, J., K. Lind and P.C. Hu. 1987. Cloning of Mycoplasma pneumoniae DNA and expression of Pl- epitopes in Escherichia coli. Isr.J.Med.Sci. 23, 759–762.PubMedGoogle Scholar
  14. 14.
    Geary, S.J., and M.G. Gabridge. 1987. Characterization of a human lung fibroblast receptor site for Mycoplasma pneumoniae. Isr.J.Med.Sci. 23, 462–468.PubMedGoogle Scholar
  15. 15.
    Gobel, U.B., M. Muller and R. Maas. 1988. Actin-related sequences of motile human mycoplasmas: a molecular puzzle. Abst. 7th int.Cong. Int.Org. Mycoplasmology, Vienna.Google Scholar
  16. 16.
    Gorski, F., and W. Bredt. 1977. Studies on the adherence mechanism of Mycoplasma pneumoniae. FEMS Microbiol. Lett. 1, 265–268.CrossRefGoogle Scholar
  17. 17.
    Hu, P.C., R.M. Cole, Y.S. Huang, J.A. Graham, D.E. Gardner, A.M. Collier, and W.A. Clyde, Jr. 1982. Mycoplasma pneumoniae infection: role of a surface protein in the attachment organelle. Science 216, 313–315.PubMedCrossRefGoogle Scholar
  18. 18.
    Hu, P.C., W.A. Clyde, Jr. and A.M. Collier. 1984. Conservation of pathogenic mycoplasma antigens. Isr. J. Med. Sci. 20, 916–919.Google Scholar
  19. 19.
    Hu, P.C., A.M. Collier, and J.B. Baseman. 1977. Surface parasitism by Mycoplasma pneumoniae of respiratory epithelium. J.Exp.Med. 145, 1328–1343.PubMedCrossRefGoogle Scholar
  20. 20.
    Hu, P.C., C.H. Huang, A.M. Collier, And W.A. Clyde, Jr. 1983. Demonstration of antibodies to Mycoplasma pneumoniae attachment protein in human sera and respiratory secretions. Infect. Immun. 41, 437–439.PubMedGoogle Scholar
  21. 21.
    Hu, P.C., C.H. Huang, Y.S. Huang, A.M. Collier, and W.A. Clyde Jr. 1985. Demonstration of multiple antigenic determinants on Mycoplasma pneumoniae attachment protein by monoclonal antibodies. Infect.Immun. 50, 292–296.PubMedGoogle Scholar
  22. 22.
    Hu, P.C., U.. Schaper, A.M. Collier, W.A. Clyde, Jr., M. Horikawa, Y.S. Huang, and M.F. Barile. 1987. A Mycoplasma genitalium protein resembling the Mycoplasma pneumoniae attachment protein. Infect. Immun. 55, 1126–1131.PubMedGoogle Scholar
  23. 23.
    Inamine, J.M., T.P. Denny, S. Loechel, U. Shaper, C.-H. Huang, K. F. Bott, and P.C. Hu. 1988. Nucleotide sequence of the Pl-attachment-protein gene of Mycoplasma pneumoniae. Gene 64, 217–229.PubMedCrossRefGoogle Scholar
  24. 24.
    Izumikawa, K., D.F. Chandler, and M.F. Barile. 1986. Mycoplasma pneumoniae attachment to glutaraldehyde- treated human WiDr cell cultures. Proc.Soc.Exp.Biol. Med. 181, 507–511.PubMedGoogle Scholar
  25. 25.
    Jacobs, E., K. Füchte, and W. Bredt. 1986. A 168-kilodalton protein of Mycoplasma pnumoniae used as antigen in a dot enzyme-linked immunosorbent assay. Eur.J.Clin.Microbiol. 5, 435–440.PubMedCrossRefGoogle Scholar
  26. 26.
    Jacobs, E., K. Füchte, and W. Bredt. 1987. Amino acid sequence and antigenicity of the amino-terminus of the 168 kDa adherence protein of Mycoplasma pneumoniae, J.Gen.Microbiol. 133, 2233–2236.PubMedGoogle Scholar
  27. 27.
    Jacobs, E., K. Schopperle, and W. Bredt. 1985. Adherence inhibition assay: a specific serological test for detection of antibodies to Mycoplasma pneumoniae. Eur.J.Clin.Microbiol. 4, 113–118.PubMedCrossRefGoogle Scholar
  28. 28.
    Kahane, I. 1984. In vitro studies on the mechanism of adherence and pathogenicity of mycoplasmas. isr.J.Med. Sci. 20, 874–877.Google Scholar
  29. 29.
    Kahane, I., S. Tucker, D.K. Leith, J. Morrison-Plummer, and J.B. Baseman. 1985. Detection of the major adhesin PI in Triton shells of virulent Mycoplasma pneumoniae. Infect. Immun. 50, 944–946.PubMedGoogle Scholar
  30. 30.
    Kanamori, M., T. Katsura, N. Ishiyama, S. Ogata, and O. Kitamoto. 1987. immune responses in Mycoplasma pneumoniae infection of infant mouse and man. isr.J. Med. Sci. 23, 568–573.PubMedGoogle Scholar
  31. 31.
    Kenny, G.E. 1979. Antigenic determinants, p. 351–384. In M.F. Barile and S. Razin (ed.), The Mycoplasmas, vol. 1: Cell Biology. Academic Press, Inc., New York.Google Scholar
  32. 32.
    Kenny, G.E. and F.D. Cartwright. 1984. Immunoblotting for determination of the antigenic specificities of antibodies to the Mycoplasmatales. Isr.J.Med.Sci. 20, 908–911.PubMedGoogle Scholar
  33. 33.
    Kirchhoff, H., R. Rosengarten, W. Lötz, M. Fischer, and D. Lopatta. 1984. Flask-shaped mycoplasmas: properties and pathogenicity for man and animals. Isr.J.Med.Sci. 20, 848–853.PubMedGoogle Scholar
  34. 34.
    Krause, D.C., D.K. Leith, R.M. Wilson, and J.B. Baseman. 1982. Identification of Mycoplasma pneumoniae proteins associated with hemadsorption and virulence, infect, immun. 35, 809–817.PubMedGoogle Scholar
  35. 35.
    Krause, D.C., D.K. Leith, and J.B. Baseman. 1983. Reacquisition of specific proteins confers virulence in Mycoplasma pneumoniae. Infect.Immun. 39, 830–836.PubMedGoogle Scholar
  36. 36.
    Kunkel, T.A., J.D. Roberts, and R.A. Zakour. 1987. Rapid and efficient site specific mutagenisis without phenotypic selection. P. 367–382. In R. Wu and L. Gross (eds.), Methods in Enzymology, vol. 154. Academic Press, Inc. Orlando, Florida.Google Scholar
  37. 37.
    Leith, D.K., and J.B. Baseman. 1984. Purification of a Mycoplasma pneumoniae adhesin by monoclonal antibody affinity chromatography. J.Bacterid. 157, 678–680.Google Scholar
  38. 38.
    Leith, D.K., L.B. Trevino, J.G. Tully, L.B. Senterfit, and J.B. Baseman. 1983. Host discrimination of Mycoplasma pneumoniae proteinaceous immunogens. J.Exp. Med. 157, 502–514.PubMedCrossRefGoogle Scholar
  39. 39.
    Lind, K. 1982. Serological cross-reactions between Mycoplasma genitalium and Mycoplasma pneumoniae. Lancet ii, 1158–1159.Google Scholar
  40. 40.
    Lind, K., B.O. Lindhardt, H.J. Schütten, J. Blom, and C. Christiansen. 1984. Serological cross-reactions between Mycoplasma genitalium and Mycoplasma pneumoniae. J.Clin.Microbiol. 20, 1036–1043.PubMedGoogle Scholar
  41. 41.
    Loomes, L.M., K. Uemura, and T. Feizi. 1985. Interaction of Mycoplasma pneumoniae with erythrocyte glycolipids of I and i antigen types. Infect.Immun. 47, 15–20.PubMedGoogle Scholar
  42. 42.
    Meng, K.E., and R.M. Pfister. 1980. Intracellular structures of Mycoplasma pneumoniae revealed after membrane removal. j.Bacteriol. 144, 390–399.PubMedGoogle Scholar
  43. 43.
    Morowitz, H.J. 1984. The completeness of molecular Biology. Isr.J.Med.Sci. 20, 750–753.PubMedGoogle Scholar
  44. 44.
    Morrison-Plummer, J., A. Lazzell, and J.B Baseman. 1987. Shared epitopes between Mycoplasma pneumoniae major adhesin protein Pi and a 140-kilodalton protein of Mycoplasma genitalium. Infect. Immun. 55, 49–56.PubMedGoogle Scholar
  45. 45.
    Morrison-Plummer, J., D.K. Leith, and J.B. Baseman. 1986. Biological effects of anti-lipid and anti-protein monoclonal antibodies on Mycoplasma pneumoniae. Infect, immun. 53, 398–403.Google Scholar
  46. 46.
    Mouches, C., T. Candresse, G. Barroso, C. Saillard, H. Wrobewski, and J.M. Bove. 1985. Gene for spiralin, the major membrane protein of the helical mollicute Spiroplasma citri:cloning and expression in Escherichia coli. J.Bacteriol. 164, 1094–1099.PubMedGoogle Scholar
  47. 47.
    Neimark, H. 1983. Mycoplasma and bacterial proteins resembling contractile proteins: a review. Yale J.Biol. Med. 56, 419–423.PubMedGoogle Scholar
  48. 48.
    Razin, S. 1978. The mycoplasmas. Microbiol.Rev. 42, 414–470.PubMedGoogle Scholar
  49. 49.
    Razin, S. 1981. The mycoplasma membrane, p. 165–250. In B.K. Ghosh (ed.). Organization of Prokaryotic Cell Membranes, vol. 1. CRC Press, Inc., Boca Raton, FL.Google Scholar
  50. 50.
    Razin, S. 1985a. Molecular biology and genetics of mycoplasmas ( Mollicutes ). Microbiol.Rev. 49, 419–455.PubMedGoogle Scholar
  51. 51.
    Razin, S. 1985b. Mycoplasma adherence, p. 161–202. In S. Razin and M.F. Barile (ed.), The Mycoplasmas, vol. 4: Mycoplasma Pathogenicity. Academic Press, Inc., Orlando, FL.Google Scholar
  52. 52.
    Razin, S. 1986. Mycoplasmal adhesins and lectins, p. 217–235. In D. Mirelman (ed.), Microbial Lectins and Agglutinins. John Wiley and Sons, Inc. New York.Google Scholar
  53. 53.
    Razin, S. and M.F. Barile (ed.). 1985. The Mycoplasmas, vol. 4: Mycoplasma Pathogenicity. Academic Press, Inc. Orlando, FL.Google Scholar
  54. 54.
    Razin, S., and J.G. Tully (ed.). 1983. Methods in Mycoplasmology, vol. 1, p. 495. Academic Press Inc. New York.Google Scholar
  55. 55.
    Razin, S., J.G. Tully, D.L. Rose, and M.F. Barile. 1983. DNA cleavage patterns as indicators of genotypic heterogeneity among strains of Acholeplasma and Mycoplasma species. J.Gen.Microbiol. 129, 1935– 1944.Google Scholar
  56. 56.
    Renaudin, J., M.C. Pascarel, C. Saillard, C.Chevalier, and J.M. Bove. 1986. In spiroplasmas, UGA is not a termination codon but seems to code for tryptophan. Comptes Rendus, Ser.III, 303, 539–540.Google Scholar
  57. 57.
    Sambrook, J.K., D.P. Fan, and S. Brenner. 1967. A strong suppressor specific for UGA. Nature, 214, 452– 454.Google Scholar
  58. 58.
    Schaper, U., J.S. Chapman, and P.C. Hu. 1987. Preliminary indication of unusual codon usage in the DNA coding sequence of the attachment protein of Mycoplasma pneumoniae. Isr.J.Med.Sci. 23, 361–367.PubMedGoogle Scholar
  59. 59.
    Su, C.J., V.V. Tryon, and J.B. Baseman. 1987. Cloning and sequence analysis of cytadhesin Pi gene from Mycoplasma pneumoniae. Infect. Immun. 55, 3023–3029.PubMedGoogle Scholar
  60. 60.
    Taylor-Robinson, D., and W. Bredt. 1983. Motility of mycoplasma strain G37. Yale J.Biol.Med. 56, 910– 911.Google Scholar
  61. 61.
    Taylor-Robinson, D., P.M. Furr, and N.F. Hanna. 1985a. Microbiological and serological study of nongonococcal urethritis with special reference to Mycoplasma genitalium. Genitourinary Med. 61, 319–324.Google Scholar
  62. 62.
    Taylor-Robinson, D., J.G. Tully, and M.F. Barile. 1985b. Urethral infection in male chimpanzees produced experimentally by Mycoplasma genitalium. Brit.J.Exp. Pathol. 66, 95–101.Google Scholar
  63. 63.
    Trevino, L.B., W.G. Haldenwang, and J.B. Baseman. 1986. Expression of Mycoplasma pneumoniae antigens in Escherichia coli. Infect. Immun. 53, 129–134.PubMedGoogle Scholar
  64. 64.
    Tully, J.G., D. Taylor-Robinson, R.M. Cole, and D.L. Rose. 1981. A newly discovered mycoplasma in the human urogenital tract. Lancet i, 1288–1291.Google Scholar
  65. 65.
    Tully, J.G., D. Taylor-Robinson, D.L. Rose, R.M. Cole, and J.M. Bove. 1983. Mycoplasma genitalium, a new species from the human urogenital tract. Int.J.Syst. Bacteriol. 33, 387–396.CrossRefGoogle Scholar
  66. 66.
    Tully, J.G., D. Taylor-Robinson, D.L. Rose, P.M. Purr, C.E. Graham, and M.F. Barile. 1986. urogenital challenge of primate species with Mycoplasma genitalium and characteristics of infection induced in chimpanzees. J.Infect. Dis. 153, 1046–1054.PubMedCrossRefGoogle Scholar
  67. 67.
    Vu, A.C., H.M. Foy, F.D. Cartwright and G.E. Kenny. 1987. The principal proteins of isolates of Mycoplasma pneumoniae measured by levels of immunoglobulin G in human serum are stable in strains collected over a 10 -year period. Infect, immun. 55, 1830–1836.Google Scholar
  68. 68.
    Woese, C.R. 1987. Bacterial evolution. Microbiol.Rev. 51, 221–271.PubMedGoogle Scholar
  69. 69.
    Yamao, F., A. Muto, Y. Kawauchi, M. Iwami, S. Iwagami, Y. Azumi, and S. Osawa. 1985. UGA is read as tryptophan in Mycoplasma capricolum. Proc.Natl.Acad.Sci. USA 82, 2306–2309.Google Scholar
  70. 70.
    Yogev, D., and S. Razin. 1986. Common deoxyribonucleic acid sequences in Mycoplasma genitalium and Mycoplasma pneumoniae genomes. Int.J.Syst.Bacteriol. 36, 426–430.CrossRefGoogle Scholar

Copyright information

© Springer-Verlag New York Inc. 1989

Authors and Affiliations

  • Shmuel Razin
    • 1
  • David Yogev
    • 1
  1. 1.Department of Membrane and Ultrastructure ResearchThe Hebrew University-Hadassah Medical SchoolJerusalemIsrael

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