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DPα Chains: 2-D Gel Patterns

  • Robert W. Knowles
  • Bernadette Kienzle
Conference paper

Abstract

The two-dimensional (2-D) gel patterns of the DPα chains for the Workshop core cell lines were examined centrally on behalf of the participants from their submitted autoradiograms. Since the DPβ chain analysis was not successful using the Workshop β chain system, the immunoprecipitates from the DP specific antibodies were not generally run on the α chain gradient. Most laboratories did, however, submit films from immunoprecipitates with the broadly reactive antibodies, including Tü39, which contained DPα chains as part of a more complex pattern including DRα chains and in some cases DQα chains. The DPα chains were also found in precipitates with other antibodies, although this was not examined systematically for all of the submitted films. The purpose of this analysis was to determine the extent of DPα chain polymorphism and whether there were significant associations with the 10th Workshop cellular DP typing of the core cell lines (1).

Keywords

Reference Panel 10th Workshop Negative Control Sample Cell Type Line Chain Pattern 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Mickelson E, Reinsmoen N, Robbins FM, Hartzman R, et al. HLA-Dw and HLA-DP typing for the reference panel of B-lymphoblastoid cell lines. In: Dupont B (ed): Immunobiology of HLA, Volume I: Histocompatibility Testing 1987. New York: Springer-Verlag, 1989.Google Scholar
  2. 2.
    Rodriguez de Cordoba S, Marshall P, Ginsberg-Fellner F, Rubinstein P. Biochemical polymorphism of the DP alpha and DP beta chains. In: Dupont B (ed): Immunobiology of HLA, Volume II: Immunogenetics and Histocompatibility. New York: Springer-Verlag, 1989.Google Scholar
  3. 3.
    Bugawan TL, Horn GT, Hansen JA, Mickelson EM, Angelini G, Ferrara GB, et al. Analysis of HLA-DP allelic sequence polymorphism using the in vitro enzymatic amplification of DP alpha and DP beta loci. In: Dupont B (ed): Immunobiology of HLA, Volume II: Immunogenetics and Histocompatibility. New York: Springer-Verlag, 1989.Google Scholar
  4. 4.
    Knowles RW. Structural polymorphism of the HLA class II α and β chains: Summary of the 10th Workshop 2-D gel analysis. In: Dupont B (ed): Immunobiology of HLA, Volume I: Histocompatibility Testing 1987. New York: Springer-Verlag, 1989.Google Scholar

Copyright information

© Springer-Verlag New York 1989

Authors and Affiliations

  • Robert W. Knowles
  • Bernadette Kienzle

There are no affiliations available

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