Population Analysis of Class I HLA Antigens by One-Dimensional Isoelectric Focusing Gel Electrophoresis: Workshop Summary Report
A total of 918 cells obtained from 58 serology laboratories were analyzed by 14 laboratories using one-dimensional isoelectric focusing gel electrophoresis (1D-IEF). This cell panel represented all ethnic groups designated by the Tenth Workshop and included 19 A locus, 35 B locus, and 8 C locus serologically defined alleles. To facilitate antigen assignment for each IEF band, two monoclonal antibodies (4E and W6/32) were used in sequential immunoprecipitations. This study demonstrated that the 1D-IEF method could define most of the serologically defined alleles and was very effective in detecting serologically indistinguishable variants or subtypes for the class I HLA antigens. The Biochemistry Class I Workshop has identified a total of 34 alleles for A locus, 52 alleles for B locus, and 11 alleles for C locus detected by 1D-IEF from this Workshop exercise. The study also demonstrated that there is an allelic consistency across different races (i.e., the same 1D-IEF alleles were frequently detected in several ethnic groups), indicating that HLA polymorphism predates the racial diversification.
KeywordsHydrate Electrophoresis Resis Methionine Valine
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