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Population Analysis of Class I HLA Antigens by One-Dimensional Isoelectric Focusing Gel Electrophoresis: Workshop Summary Report

  • Soo Young Yang

Abstract

A total of 918 cells obtained from 58 serology laboratories were analyzed by 14 laboratories using one-dimensional isoelectric focusing gel electrophoresis (1D-IEF). This cell panel represented all ethnic groups designated by the Tenth Workshop and included 19 A locus, 35 B locus, and 8 C locus serologically defined alleles. To facilitate antigen assignment for each IEF band, two monoclonal antibodies (4E and W6/32) were used in sequential immunoprecipitations. This study demonstrated that the 1D-IEF method could define most of the serologically defined alleles and was very effective in detecting serologically indistinguishable variants or subtypes for the class I HLA antigens. The Biochemistry Class I Workshop has identified a total of 34 alleles for A locus, 52 alleles for B locus, and 11 alleles for C locus detected by 1D-IEF from this Workshop exercise. The study also demonstrated that there is an allelic consistency across different races (i.e., the same 1D-IEF alleles were frequently detected in several ethnic groups), indicating that HLA polymorphism predates the racial diversification.

Keywords

Donor Cell American Indian Bold Type Panel Cell Serologic Specificity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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© Springer-Verlag New York 1989

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  • Soo Young Yang

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