RFLP Standardization Report for DP Beta/EcoRI

Abstract

The majority of the 14 fragments identified in the DP beta/EcoRI system were cross-hybridizing with DRB and DQB (Table 3 in the DR Beta/EcoRI report). The DP beta–specific bands were numbers 2,6,7, and 12 (Table 1 and Fig. 1), the latter being a constant band. Six different banding patterns were also identified. Only fragment number 7 (7.20 kb) was significantly correlated with a specific DP type (DPw2; x²=15.166). EcoRI appears to have limited value in DP typing using RFLP. The discordance between hidden duplicates in this system (fragment 13) was caused by lack of resolution of close bands.

Participating Laboratories: UKIBOD,¹ FRABET,² UKIBID,³ US4GVY,⁴ UKIBAT,⁵ JAPNAI⁶