RFLP Standardization Report for DP Beta/BgIII
Data were received from five different laboratories and standardization was performed based on the following criteria: 1) weak hybridization signals were accepted as standard fragments when they were detected by most laboratories, and 2) discrepancies were resolved by majority consensus. For this system, 18 different standard fragments were observed (Table 1 and in Fig. 1). The first standard fragment has a molecular weight of 20.50 kb and a panel frequency of 100%. This fragment appears to be HLA-DP-specific. The second standard fragment has a molecular weight of 17.49 kb and is observed in 71.4% of the cells tested. This fragment is also detected in the DR beta/BGIII system as standard fragment 1. Standard fragment 3 appears to be HLA-DP-specific and is observed with a panel frequency of 78.6%. This fragment is always present in an abundant staining intensity (Fig. 1). Standard fragments 4 and 5 are also detected with a DR beta probe (see DR Beta/BgIII report Table 2) and have a molecular weight of 1 1.58 and 10.18 kb, respectively. Standard fragment 6 has a molecular weight of 9.38 kb and is also detected with DR and DQ beta probes (see DR Beta/BgIII report Table 2). In contrast to this, standard fragment 7 with a molecular weight of 8.99 kb is detected both with DR and DP beta probes, but not with a DQ beta probe (see DR Beta/BgIII report Table 2). Fragment 8 seems to be HLA-DP specific and has a molecular weight of 7.28 kb (Table 1). The same situation that is observed for standard fragment 6 is also noticed for standard fragment 9 (Table 2). Standard fragment 10 appears to be DP- specific and has a molecular weight of 6.56 kb and a panel frequency of 17.1%. Again, fragment 11, like fragments 6, 9, 16, and 17, are also detected within the DR beta/BgIII and DQ beta/BgIII systems (see DR beta/BgIII report Table 2).