RFLP Standardization Report for DQ Beta/EcoRI

Abstract

The DQ beta/EcoRI system identified 13 fragments in the core cell lines (Fig. 1). Locus assignments in this system (Table 1) showed fragments 1,2,4,5,6,10, & 13 to be DQB specific, the remaining fragments cross-hybridizing with both DRB and DPB (Table 3 in the DR Beta/EcoRI report). A number of different banding patterns were revealed in this system, standard fragment 6 being a faint constant band. All the other unique bands were found to be polymorphic.

Participating Laboratories: UKIBOD,¹ FRABET,² UKIBID,³ US4GVY,⁴ UKIBAT,⁵ JAPNAI⁶