Immunological Characterization of a Viral Agent Involved in Epidemic and Sporadic Non-A Non-B Hepatitis

  • J. Pillot
  • M. D. Sharma
  • Y. Lazizi
  • A. Budkowska
  • C. Dauguet
  • J. L. Sarthou
Conference paper
Part of the Progress in Vaccinology book series (VACCINOLOGY, volume 2)


In humans, acute and/or chronic hepatitis are due to virus A, B, δ, and others not yet known. A virus possibly involved in epidemic and sporadic cases of non-A non-B (NANB) hepatitis has been isolated. This virus is infectious for African green and Saimiri monkeys, and the antibodies pro-duced in these nonhuman primates have been used to develop an ELISA detecting a NANB-associated antigen in stool. This virus grows in the hepatocyte PLC/PRF 5 cell line but not in HEP G2. Two types of particle were observed in association with the NANB antigen: large particles of 60 to 80 nm diameter (presumed complete virions), and smaller, lighter homogeneous particles of 25 to 30 nm. Using the inhibition of ELISA, antibodies have been detected in sera from African patients with epidemic or endemic NANB hepatitis as well as from European patients with en-demic NANB hepatitis.


Ivory Coast Stool Specimen Viral Agent Immunological Characterization NANB Hepatitis 
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  1. 1.
    Andjaparidze AG, Balayan MS, Savinov AP, et al: Non A non B hepatitis transmitted by the fecal oral mode experimentally produced in monkeys. Vopr Virusol 1986; 31: 73–81.Google Scholar
  2. 2.
    Balayan MS, Andjaparidze AG, Savinska SS, et al: Evidence for a virus in non A non B hepatitis transmitted via fecal oral route. Intervirology 1983;20:23– 31.Google Scholar
  3. 3.
    Belabbes EH, Bouguermough A, Bentallah A, Illoul G: Epidemic non A non B viral hepatitis in Algeria: strong evidence for its spreading by water. J Med Virol 1985; 16: 275–281.CrossRefGoogle Scholar
  4. 4.
    Francis DP, Hadler SC, Prendergast TJ: Occurrence of hepatitis A, B and non A non B in the United States: CDC Sentinel County hepatitis study. Am J Med 1984; 76: 69–74.PubMedCrossRefGoogle Scholar
  5. 5.
    Kane MA, Bradley DW, Shrestha SM, et al: Epidemic non A non B hepatitis in Nepal: recovery of a possible etiologic agent and transmission in marmosets. JAMA 1984; 252: 3140–3145.PubMedCrossRefGoogle Scholar
  6. 6.
    Khuroo MS: Study of an epidemic of non A non B hepatitis: possibility of another human hepatitis virus distinct from post-transfusion non A non B type. Am J Med 1980; 68: 818–824.PubMedCrossRefGoogle Scholar
  7. 7.
    Molinie C, Roue E, Saliou P, et al: Hépatite aiguë non A non B epidémique: étude clinique de 38 cas observés au Tchad. Gastroenterol Clin Biol 1986; 10: 475–479.PubMedGoogle Scholar
  8. 8.
    Petit MA, Capel F, Pillot J: Demonstration of a firm association between hep¬atitis B surface antigen proteins bearing polymerized human albumin binding sites and core-specific determinants in serum hepatitis B viral particles. Mol Immunol 1985; 22: 1279–1287.PubMedCrossRefGoogle Scholar
  9. 9.
    Pillot J, Petit MA: Immunochemical structure of the hepatitis B surface antigen vaccine. I. Treatment of immobilized HBsAg by dissociation agents with or without enzymatic digestion and identification of polypeptides by protein blot¬ting. Mol Immunol 1984; 21: 53–60.PubMedCrossRefGoogle Scholar
  10. 10.
    Prince AM : Non A non B hepatitis viruses. Ann Rev Microbiol 1983;37:217– 232CrossRefGoogle Scholar
  11. 11.
    Sarthou JL, Budkowska A, Sharma MD, et al: Characterization of an antigen- antibody system associated with epidemic non A non B hepatitis in West Africa and experimental transmission of an infectious agent to primates. Ann Inst Pasteur Virol 1986; 137E: 225–232.CrossRefGoogle Scholar
  12. 12.
    Shiraishi H, Alter HJ, Feinstone SM, Purcell RH: Rheumatoid factor-like reactants in sera proven to transmit non A non B hepatitis: a potential source of false positive reactions in non A non B assays. Hepatology 1985;5:181–187PubMedCrossRefGoogle Scholar
  13. 13.
    Sreenivasan MA, Arankale VA, Seghal A, Pavri KM: Non A non B epidemic hepatitis: vizualization of virus-like particles in the stools by immune electron microscopy. J Gen Virol 1984; 65: 1005–1007.PubMedCrossRefGoogle Scholar
  14. 14.
    Yamauchi M, Nakajima H, Kimura K, et al: An epidemic of non A non B hepatitis in Japan. Am J Gastroenterol 1983; 78: 652–655.PubMedGoogle Scholar

Copyright information

© Springer-Verlag New York Inc. 1989

Authors and Affiliations

  • J. Pillot
  • M. D. Sharma
  • Y. Lazizi
  • A. Budkowska
  • C. Dauguet
  • J. L. Sarthou

There are no affiliations available

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