Expression of the Potyvirus Genome: The Role of Proteolytic Processing
The potato virus Y [potyvirus] group is comprised of a number of distinctive members, estimated to be between 100 and 150 dependent on the criteria used in classification (Edwardson, 1974; Hollings and Brunt, 1981). Although each member has a rather restrictive host range, the large number of putative members make these viruses ubiquitous pathogens present in most agricultural settings. As such, over the past 5 years they have become one of the more intensively studied plant virus groups. Using molecular biological approaches, our understanding of potyvirus gene structure, function, and expression has increased dramatically.
KeywordsCleavage Site Potato Virus Tobacco Etch Virus Soybean Mosaic Virus Nuclear Inclusion
Unable to display preview. Download preview PDF.
- Allison, R.F., Sorenson, J.C., Kelly, M.E., Armstrong, F.B., and Dougherty, W.G. (1984). Sequence determination of the capsid protein gene and flanking regions of tobacco etch virus: evidence for the synthesis of a polyprotein in potyvirus genome expression. Proc. Natl. Acad. Sci. USA 82: 3969–3972.CrossRefGoogle Scholar
- Carrington, J.C., Freed, D.D., and Sanders, T.C. (1989b). Autocatalytic processing of the potyvirus helper component proteinase in Escherichia coli and in vitro. J. of Virology 63: 4459–4463.Google Scholar
- Christie, R.G., and Edwardson, J.R. (1977). Light and electron microscopy of plant virus inclusions. Fla Agric. Exp. Sta. Monogr. Ser. 9.198pp.Google Scholar
- Dougherty, W.G., and Hiebert, E. (1980a). Translation of potyviral RNA in a rabbit reticulocyte lysate: identification of nuclear inclusion protein as products of tobacco etch virus RNA translation and cytoplasmic inclusion protein as a product of the potyvirus genome. Virology 104, 174–182.PubMedCrossRefGoogle Scholar
- Edwardson, J.R. (1974). Some properties of the potato virus Y-group. Fla. Agric. Exp. Sta. Monogr. Ser. 4.398pp.Google Scholar
- Hiebert, E., Purcifull, D., and Christie, R.C. (1984). Purification and immunological analysis of plant viral inclusion bodies. Methods in Virology 7: 225–280., Eds. K. Maramorosh and H. Koprowski. Academic Press, N.Y.Google Scholar
- Hollings, M., and Brunt, A.A. (1981). Potyviruses. in “Handbook of Plant Virus Infections and Comparative Diagnosis”. (Ed. E. Kurstak). pp701–754. Elsevier/North-Holland Biomedical, New York.Google Scholar
- Murphy, J., Shaw, J.G., and Rhoads, R.E. (1989). The VPg of tobacco etch virus. Phytopathology 79: 1214 (Abst.)Google Scholar
- Oh, C.-S., and Carrington, J.C. (1989). Identification of essential residues in potyvirus proteinase HC-PRO by site-directed mutagenesis. Virology. in press.Google Scholar
- Slade, D.E., Johnston, R.E., and Dougherty, W.G. (1989). Generation and characterization of monoclonal antibodies reactive with the 49kDa proteinase of tobacco etch virus. Virology 173: in press.Google Scholar