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Microbial Ectoenzymes in Aquatic Environments

  • Ryszard J. Chróst
Part of the Brock/Springer Series in Contemporary Bioscience book series (BROCK/SPRINGER)

Abstract

During the past decade an increasing number of ecological studies have considered the complexity of aquatic environments. One major outcome of these studies has been an accelerated interest in the role of microheterotrophs and the mode by which organic matter is made available to them. The heterotrophic microorganisms are the key level at which the metabolism of the whole ecosystem is affected, i.e., nutrient cycling, organic matter transformation and mineralization, and energy flow. The measurement of microbial activity in natural waters is very important for understanding the dynamic aspects of the functioning of the whole ecosystem.

Keywords

Aquatic Environment Lake Water Alkaline Phosphatase Activity Cytoplasmic Membrane Natural Substrate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Aaronson, S. 1981. Chemical Communication at the Microbial Level vol. 1. CRC Press, Inc., Boca Raton, 184 pp.Google Scholar
  2. Aaronson, S. and Patni, N.J. 1976. The role of surface and extracellular phosphatases in the phosphorus requirement of Chromonas Limnology and Oceanography 21: 838–845.Google Scholar
  3. Aizawa, K. and Miyachi, S. 1986. Carbonic anhydrase and CO2 concentrating mechanisms in microalgae and cyanobacteria. Federation of European Microbiology Societies, Microbiology Review 39: 215–233.CrossRefGoogle Scholar
  4. Ammerman, J.W. and Azam, F. 1985. Bacterial 5’-nucleotidase in aquatic ecosystems: A novel mechanism of phosphorus regeneration. Science 227: 1338–1340.PubMedCrossRefGoogle Scholar
  5. Armstrong, F.B. 1983. Biochemistry, 2nd edition. Oxford University Press, New York, 653 pp.Google Scholar
  6. Azam, F. and Cho, B.C. 1987. Bacterial utilization of organic matter in the sea. pp. 261–281 in Fletcher, M., Gray, T.R.G., and Jones, J.G. (editors), Ecology of Microbial Communities. Cambridge University Press, Cambridge.Google Scholar
  7. Bengtsson, G. 1988. The impact of dissolved amino acids on protein and cellulose degradation in stream waters. Hydrobiologia 164: 97–102.CrossRefGoogle Scholar
  8. Berg, H.C. 1969. Sulphanilic acid diazonium salt: A label for the outside of the human erythrocyte membrane. Biochimica et Biophysica Acta 183: 65–78.PubMedCrossRefGoogle Scholar
  9. Berman, T. 1970. Alkaline phosphatases and phosphorus availability in Lake Kinneret. Limnology and Oceanography 15: 663–674.CrossRefGoogle Scholar
  10. Berry, R.K. and Dekker, R.F.H. 1984. Induction studies showing evidence of the similarities between an inducible intracellular and extracellular β-D-glucosidase produced by a species of Monilia. Federation of European Microbiology Societies, Microbiology Letters 21: 309–312.Google Scholar
  11. Blobel, G., Walter, P., Chang, C.N., Goldman, B.M., Erickson, A.H., and Lingappa, V.R. 1979. Translation of proteins across membranes: the signal hypothesis and beyond. Symposium of Society of Experimental Biology 33: 9–36.Google Scholar
  12. Botsford, J.L. 1981. Cyclic nucleotides in prokaryotes. Microbiological Reviews 45: 620–645.PubMedGoogle Scholar
  13. Burns, R.G. 1983. Extracellular enzyme—substrate interactions in soil. pp. 249–298 in Slater, J.H., Whittenbury, R., and Wimpenny, J.W.T. (editors), Microbes in Their Natural Environments. Cambridge University Press, London.Google Scholar
  14. Chaloupka, J. and Krumphanzl, V. 1987. Extracellular Enzymes of Microorganisms. Plenum Press, New York, 216 pp.Google Scholar
  15. Chróst, R.J. 1984. Use of 14C-dissolved organic carbon (RDOC) released by algae as a realistic tracer for heterotrophic activity measurements for aquatic bacteria. Archiv für Hydrobiologie, Ergebnisse der Limnologie 19: 207–214.Google Scholar
  16. Chróst, R.J. 1986. Algal—bacterial metabolic coupling in the carbon and phosphorus cycle in lakes, pp. 360–366 in Megusar, F., and Gantar, M. (editors), Perspectives in Microbial Ecology. Slovene Society of Microbiology, Ljubljana.Google Scholar
  17. Chróst, R.J. 1988. Phosphorus and microplankton development in an eutrophic lake. Acta Microbiologica Polonica 37: 205–225.Google Scholar
  18. Chróst, R.J. 1989. Characterization and significance of β-glucosidase activity in lake water. Limnology and Oceanography 34: 660–672.CrossRefGoogle Scholar
  19. Chróst, R.J. and Faust, M.A. 1983. Organic carbon release by phytoplankton: its composition and utilization by bacterioplankton. Journal of Plankton Research 5: 477–493.CrossRefGoogle Scholar
  20. Chróst, R.J. and Krambeck, H.J. 1986. Fluorescence correction for measurements of enzyme activity in natural waters using methylumbelliferyl-substrates. Archiv für Hydrobiologie 106: 79–90.Google Scholar
  21. Chróst, R.J. and Overbeck, J. 1987. Kinetics of alkaline phosphatase activity and phosphorus availability for phytoplankton and bacterioplankton in lake Plußsee (north German eutrophic lake). Microbial Ecology 13: 229–248.CrossRefGoogle Scholar
  22. Chróst, R.J., Siuda, W., and Halemejko, G.Z. 1984. Longterm studies on alkaline phosphatase activity (APA) in a lake with fish-aquaculture in relation to lake eutrophication and phosphorus cycle. Archiv für Hydrobiologie, Supplement 70: 1–32.Google Scholar
  23. Chróst, R.J., Halemejko, G.Z., and Overbeck, J. 1986a. Is proteolysis dependent on phosphorus in freshwaters? Federation of European Microbiology Societies, Microbiology Letters 37: 199–202.CrossRefGoogle Scholar
  24. Chróst, R.J., Wcislo, R., and Halemejko, G.Z. 1986b. Enzymatic decomposition of organic matter by bacteria in an eutrophic lake. Archiv für Hydrobiologie 107: 145–165.Google Scholar
  25. Chróst, R.J., Siuda, W., Albrecht, D., and Overbeck, J. 1986c. A method for determining enzymatically hydrolyzable phosphate (EHP) in natural waters. Limnology and Oceanography 31: 662–667.CrossRefGoogle Scholar
  26. Chróst, R.J., Münster, U., Rai, H., Albrecht, D., Witzel, P.K., and Overbeck, J. 1989. Photosynthetic production and exoenzymatic degradation of organic matter in euphotic zone of an eutrophic lake. Journal of Plankton Research 11: 223–242.CrossRefGoogle Scholar
  27. Critchley, C. and Andrews, T.J. 1984. Photosynthesis and plasmalemma permeability properties of Prochloron. Archives of Microbiology 138: 247–250.CrossRefGoogle Scholar
  28. Cunningham, H.W. and Wetzel, R.G. 1989. Kinetic analysis of protein degradation by a freshwater wetland sediment community. Applied and Environmental Microbiology 55: 1963–1967.PubMedGoogle Scholar
  29. Darnell, Jr. J.E. 1982. Variety in the level of gene control in eukaryotic cells. Nature 297: 365–371.PubMedCrossRefGoogle Scholar
  30. Davis, B.D. and Tai, P.C. 1980. The mechanism of protein secretion across membranes. Nature 283: 433–438.PubMedCrossRefGoogle Scholar
  31. Deason, T.R. 1983. Cell wall structure and composition as taxonomic charcters in the coccoid Chlorophyceae Journal of Phycology 19: 248–251.CrossRefGoogle Scholar
  32. DePierre, J.W. and Karnovsky, M.L. 1974. Ecto-enzymes of the guinea-pig polymorphonuclear leukocyte. II. Properties and suitability as markers for the plasma membrane. Journal of Biological Chemistry 249: 7121–7129.PubMedGoogle Scholar
  33. Dowd, J.E. and Riggs, D.S. 1965. A comparison of estimates of Michaelis-Menten kinetic constants from various linear transformations. Journal of Biological Chemistry 240: 863–869.PubMedGoogle Scholar
  34. Drews, G. 1973. Fine structure and chemical composition of the cell envelopes, pp. 99–116 in Carr, N.G., and Whitton, B.A. (editors), The Biology of Blue-Green Algae. Blackwell, Oxford.Google Scholar
  35. Drews, G. and Giesbrecht, P. 1971. Die Bauelemente der Bakterien und Blaualgen, pp. 407–467 in Metzner, H. (editor), Die Zelle. Wissenschaftliche Verlagsgesellschaft, Stuttgart.Google Scholar
  36. Fenchel, T. 1987. Ecology of Protozoa. Science Tech., Madison, 193 pp.Google Scholar
  37. Francko, D. 1984. Phytoplankton metabolism and cyclic nucleotides. II. Nucleotide-induced perturbations of alkaline phosphatase activity. Archiv für Hydrobiologie 100: 409–421.Google Scholar
  38. Frankenberger, W.T. and Johanson, A.J.B. 1983. Amidohydrolase activity in natural waters. Polskie Archiwum Hydrobiologii 30: 319–329.Google Scholar
  39. Frankenberger, W.T. and Johanson, A.J.B. 1986. Use of plasmolytic agents and antiseptics in soil enzyme assays. Soil Biology and Biochemistry 18: 209–214.CrossRefGoogle Scholar
  40. Glenn, A.R. 1976. Production of extracellular proteins by bacteria. Annual Reviews of Microbiology 30: 41–62.CrossRefGoogle Scholar
  41. Halemejko, G.Z. and Chróst, R.J. 1984. The role of phosphatases in phosphorus mineralization during decomposition of lake phytoplankton blooms. Archiv für Hydrobiologie 101: 489–502.Google Scholar
  42. Halemejko, G.Z. and Chróst, R.J. 1986. Enzymatic hydrolysis of proteinaceous particulate and dissolved material in an eutrophic lake. Archiv für Hydrobiologie 107:1–21.Google Scholar
  43. Hollibaugh, J.T. and Azam, F. 1983. Microbial degradation of dissolved proteins in seawater. Limnology and Oceanography 28: 1104–1116.CrossRefGoogle Scholar
  44. Hoppe, H.G. 1983. Significance of exoenzymatic activities in the ecology of brackish water: measurements by means of methylumbelliferyl-substrates. Marine Ecology Progress Series 11: 299–308.CrossRefGoogle Scholar
  45. Hoppe, H.G. 1986. Degradation in sea water, pp. 453–474 in Rehm, H.J. and Reed, G. (editors), Biotechnology. Vol. 8. VCH Verlagsgesellschaft, Weinheim.Google Scholar
  46. Hoppe, H.G., Kim, S.J., and Gocke, K. 1988. Microbial decomposition in aquatic environments: combined processes of extracellular enzyme activity and substrate uptake. Applied and Environmental Microbiology 54: 784–790.PubMedGoogle Scholar
  47. Hoppe, H.G., Gocke, K., Zamorano, D., and Zimmermann, R. 1983. Degradation of macromolecular organic compounds in a tropical lagoon (Cienaga Grande, Colombia) and its ecological significance. Internationale Revue gesamten Hydrobiologie 68: 811–824.CrossRefGoogle Scholar
  48. Imanaka, T. Tanaka, T., Tsunekawa, H. and Aiba, S. 1981. Cloning of the genes for penicillinase, penP and penI, of Bacillus licheniformis in some vector plasmids and their expression inEscherichia coli, Bacillus subtilis and Bacillus licheniformis. Journal of Bacteriology 147: 776–786.PubMedGoogle Scholar
  49. Inouye, M. and Halegoua, S. 1980. Secretion, and membrane localization of proteins in Escherichia coli. CRC Critical Reviews in Biochemistry 7: 339–371.PubMedCrossRefGoogle Scholar
  50. Jacobsen, T.R. and Azam, F. 1985. Role of bacteria in copepod fecal pellet decomposition: colonization, growth rates and mineralization. Bulletin of Marine Sciences 35: 495–502.Google Scholar
  51. Jacobsen, T.R. and Rai, H. 1988. Determination of aminopeptidase activity in lakewater by a short term kinetic assay and its application in two lakes of differing eutro-phication. Archiv für Hydrobiologie 113: 359–370.Google Scholar
  52. Jansson, M., Olsson, H. and Broberg, O. 1981. Characterization of acid phosphatases in the acidified lake Gardsjon, Sweden. Archiv für Hydrobiologie 92: 377–395.Google Scholar
  53. Jones, J.G. 1972. Studies on freshwater microorganisms: phosphatase activity in lakes of differing degrees of eutrophication. Journal of Ecology 60: 777–791.CrossRefGoogle Scholar
  54. Kalisz, H.M. 1988. Microbial proteinases, pp. 1–66 in Fiechter, A. (editor), Advances in Biochemical Engineering/-Biotechnology. Enzyme Studies, vol. 36. Springer Verlag, New York.Google Scholar
  55. Karnovsky, M.L. 1986. Ectoenzymes: their modulation and similarity to certain enzymes of intracellular membranes, pp. 3–13 in Kreutzberg, G.W., Reddington, M., and Zimmermann, H. (editors), Cellular Biology of Ectoenzymes. Springer Verlag, Berlin.Google Scholar
  56. King, G.M. 1986. Characterization of β-glucosidase activity in intertidal marine sediments. Applied and Environmental Microbiology 51: 373–380.PubMedGoogle Scholar
  57. King, G.M. and Klug, M.J. 1980. Sulfhydrolase activity in sediments of Wintergreen Lake, Kalamazoo County, Michigan. Applied and Environmental Microbiology 39: 950–956.PubMedGoogle Scholar
  58. Kobori, H., Taga, N. and Simidu, U. 1979. Properties and generic composition of phosphatase producing bacteria in coastal and oceanic waters. Bulletin of Japanese Society of Scientific Fisheries 45: 1429–1433.CrossRefGoogle Scholar
  59. Kreil, G. 1981. Transfer of proteins across membranes. Annual Reviews of Biochemistry 50: 317–348.CrossRefGoogle Scholar
  60. Leatherbarrow, R.J. 1987. Enzfitter. A Non-linear Regression Data Analysis Program for the IBM PC. Elsevier-Biosoft, Cambridge, pp. 91.Google Scholar
  61. Little, J.E., Sjogren, R.E., and Carson, G.R. 1979. Measurement of proteolysis in natural waters. Applied and Environmental Microbiology 37: 900–908.PubMedGoogle Scholar
  62. Lochte, M.A. and Ford, T.E. 1986. Metabolism of dissolved organic matter by attached microorganisms in rivers, pp. 367–374 in Megusar, F. and Gantar, M. (editors), Perspectives in Microbial Ecology. Slovene Society of Microbiology, Ljubljana.Google Scholar
  63. Lundin, A., Arner, P., and Hellmer, J. 1989. A new linear plot for standard curves in kinetic substrate assays extended above the Michaelis-Menten constant: application to a luminometric assay of glycerol. Analytical Biochemistry 177: 125–131.PubMedCrossRefGoogle Scholar
  64. Maeda, M. and Taga, N. 1973. Deoxiribonuclease activity in seawater and sediment. Marine Biology 20: 58–63.CrossRefGoogle Scholar
  65. McConahey, P.J. and Dixon, F.J. 1966. A method for trace iodination of proteins for immunological studies. International Revue of Allergy and Applied Immunology 29: 185–189.CrossRefGoogle Scholar
  66. Meyer, D.H. 1976. Secretion of β-glucosidase by Ochromonas danica. Archives of Microbiology 109: 263–270.CrossRefGoogle Scholar
  67. Meyer, D.I., Krause, E., and Dobberstein, B. 1982. Secretory protein translocation across membrane—role of the “docking protein”. Nature297: 647–650.PubMedCrossRefGoogle Scholar
  68. Meyer-Reil, L.A. 1981. Enzymatic decomposition of proteins and carbohydrates in marine sediments: methodology and field observations during spring. Kieler Meeresforschungen 5: 311–317.Google Scholar
  69. Meyer-Reil, LA. 1986. Measurement of hydrolytic activity and incorporation of dissolved organic substrates by microorganisms in marine sediments. Marine Ecology Progress Series 31: 143–149.CrossRefGoogle Scholar
  70. Meyer-Reil, L.A. 1987. Seasonal and spatial distribution of extracellular enzymatic activities and microbial incorporation of dissolved organic substrates in marine sediments. Applied and Environmental Microbiology 53: 1748–1755.PubMedGoogle Scholar
  71. Molano, J., Duran, A., and Cabib, E. 1977. A rapid and sensitive assay for chitinase using tritiated chitin. Annales of Biochemistry 83: 648–656.CrossRefGoogle Scholar
  72. Münster, U. 1984. Distribution, dynamic and structure of free dissolved carbohydrates in the Plußsee, a North German eutrophic lake. Internationale Vereinigung für Theoretische und Angewandte Limnologie, Verhandlungen 22: 929–935.Google Scholar
  73. Münster, U. 1985. Investigations about structure, distribution and dynamics of different organic substrates in the DOM of lake Plußsee. Archiv für Hydrobiologie, Supplement 70: 429–480.Google Scholar
  74. Münster, U., Einio, P., and Nurminen, J. 1989. Evaluation of the measurements of extracellular enzyme activities in a polyhumic lake by means of studies with 4-methylumbelliferyl-substrates. Archiv für Hydrobiologie 115: 321–337.Google Scholar
  75. Nikaido, H. and Nakae, T. 1979. The outer membrane of Gram-negative bacteria. Advances of Microbial Physiology 20: 163–250.CrossRefGoogle Scholar
  76. Nisbet, B. 1984. Nutrition and Feeding Strategies in Protozoa. Croom Helm, London, 156 pp.Google Scholar
  77. Olsson, H. 1983. Origin and production of phosphatases in the acid lake Gardsjon. Hydrobiologia 101: 49–58.CrossRefGoogle Scholar
  78. Pace, M.L. 1988. Bacterial mortality and the fate of bacterial production. Hydrobiologia 159: 41–49.CrossRefGoogle Scholar
  79. Paul, J.H., Jeffrey, W.H., and DeFlaun, M.F. 1987. Dynamics of extracellular DNA in the marine environment. Applied and Environmental Microbiology 53: 170–179.PubMedGoogle Scholar
  80. Perry, M.J. 1972. Alkaline phosphatase activity in subtropical Central North Pacific waters using a sensitive fluorometric method. Marine Biology 15: 113–119.CrossRefGoogle Scholar
  81. Petterson, K. 1980. Alkaline phosphatase activity and algal surplus phosphorus as phosphorus deficiency indicators in Lake Erken. Archiv für Hydrobiologie 89: 54–87.Google Scholar
  82. Pollock, M.R. 1962. Exoenzymes. pp. 121–178 in Gunsalus, I.C., and Stanier, R.Y. (editors), The Bacteria. Vol. 4. Academic Press, New York.Google Scholar
  83. Pomeroy, L.R. and Wiebe, W.J. 1988. Energetics of microbial food webs. Hydrobiologia 159: 7–18.CrossRefGoogle Scholar
  84. Prats, M. and Forestier, J.P. 1988. A new approach to Michaelis-Menten kinetics and enzyme inhibition. Biochemical Education 16: 217–221.CrossRefGoogle Scholar
  85. Priest, F.G. 1977. Extracellular enzyme synthesis in the genus Bacillus. Bacteriological Reviews 41: 711–753.Google Scholar
  86. Priest, F.G. 1984. Extracellular Enzymes. Van Nostrand Reinhold (UK) Co. Ltd., Wokingham, 79 pp.Google Scholar
  87. Rego, J.V., Billen, G., Fontigny, A., and Somville, M. 1985. Free and attached proteolytic activity in water environments. Marine Ecology Progress Series 21: 245–249.CrossRefGoogle Scholar
  88. Reichardt, W. 1971. Catalytic mobilization of phosphate in lake water and by Cyano-phyta. Hydrobiologia 38: 377–394.CrossRefGoogle Scholar
  89. Reichardt, W., Overbeck, J., and Steubing, L. 1967. Free dissolved enzymes in lake waters. Nature 216: 1345–1347.CrossRefGoogle Scholar
  90. Rice, R.H. and Means, G.E. 1971. Radioactive labelling of proteins in vitro. Journal of Biological Chemistry 246: 831–832.PubMedGoogle Scholar
  91. Rogers, H.J. 1961. The dissimilation of high molecular weight organic substrates, pp. 261–318 in Gunsalus, I.C., and Stanier, R.Y. (editors), The Bacteria. Vol. 2. Academic Press, New York.Google Scholar
  92. Rogers, H.J., Perkins, H.R., and Ward, J.B. 1980. Microbial Cell Wall and Membranes. Chapman and Hall, London, 367 pp.Google Scholar
  93. Roso, A.L. and Azam, F. 1987. Proteolytic activity in coastal oceanic waters: depth distribution and relationship to bacterial populations. Marine Ecology Progress Series 41: 231–240.CrossRefGoogle Scholar
  94. Savageau, M.A. 1979. Autogenous and classical control of gene expression: a general theory and experimental evidence, pp. 57–108 in Goldberg, R.F. (editor), Biological Regulation and Develpment. Vol. 1, Gene Expression. Plenum Press, New York.Google Scholar
  95. Scherrer, R. and Gerhardt, P. 1971. Molecular sieving by the Bacillus megaterium cell wall and protoplast. Journal of Bacteriology 107: 718–735.PubMedGoogle Scholar
  96. Schneider, Y.J., Tulkens, D., deDuve, D., and Trouet, A. 1979. Fate of plasma membrane during endocytosis. II. Evidence for recycling (shuttle) of plasma membrane constituents. Journal of Cellular Biology 82: 380–387.CrossRefGoogle Scholar
  97. Siuda, W. 1984. Phosphatases and their role in organic phosphorus transformation in natural waters. A review. Polskie Archiwum Hydrobiologii 31: 207–233.Google Scholar
  98. Siuda, W. and Chrost, R.J. 1987. The relationship between alkaline phosphatase (APA) activity and phosphate availability for phytoplankton and bacteria in eutrophic lakes. Acta Microbiologica Polonica 36: 247–257.Google Scholar
  99. Smucker, R.A. and Kim, C.K. 1987. Chitinase induction in an estuarine system, pp. 347–355 in Llevellyn, G.C., and O’Rear, C.O. (editors), Biodeterioration Research. Plenum Press, New York.Google Scholar
  100. Somville, M. 1984. Measurement and study of substrate specificity of exoglucosidase activity in eutrophic water. Applied and Environmental Microbiology 48:1181–1185.PubMedGoogle Scholar
  101. Somville, M. and Billen, G. 1983. A method for determining exoproteolytic activity in natural waters. Limnology and Oceanography 28: 190–193.CrossRefGoogle Scholar
  102. Stevens, R.J. and Parr, M.P. 1977. The significance of alkaline phosphatase activity in Lough Neagh. Freshwater Biology 7: 351–355.CrossRefGoogle Scholar
  103. Verner, K. and Schatz, G. 1988. Protein translocation across membranes. Science 241: 1307–1313.PubMedCrossRefGoogle Scholar
  104. Verstraeke, W., Voets, J.P., and van Lancker, P. 1976. Evaluation of some enzymatic methods to measure the bioactivity of aquatic environments. Hydrobiologia 49: 257–266.CrossRefGoogle Scholar
  105. Wickner, W. 1979. The asembly of proteins into biological membranes: the membrane trigger hypothesis. Annual Reviews of Biochemistry 48: 23–45.CrossRefGoogle Scholar
  106. Williams, P.J.LeB. 1981. Incorporation of microheterotrophic processes into the classical paradigm of the planktonic food web. Kieler Meeresforschungen, Sonderheft 5: 1–28.Google Scholar

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  • Ryszard J. Chróst

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