Abstract
The advent of directed mutagenesis has made it possible to alter protein structures at will. For the first time it is possible to design and to introduce modifications into a protein that are intended to change its behavior in predictable ways.
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References
Alber, T., Dao-pin, S., Wilson, K. Wozniak, J.A., Cook, S.P. and Matthews, B.W. (1987) Nature 330, 41–46.
Alber, T., Grötter, M.G., Gray, T.M., Wozniak, J., Weaver, L.H., Chen, BL., Baker, E.N. and Matthews, B.W. (1986) UCLA Symposium on Molecular and Cellular Biology, New Series, D.L. Oxender, ed. Alan R. Liss, Inc., NY 39, 307–318.
Baker, E.N. and Hubbard, R.E. (1984) Prog. Biophys. Molec. Biol. 44, 97–179.
Bell, J.A., Dao-pin, S., Faber, R., Jacobson, R., Karpusas, M., Matsumura, M., Nicholson, H., Pjura, P.E., Tronrud, D.E., Weaver, L.H., Wilson, K., Wozniak, J.A., Zhang, X-J., Alber, T. and Matthews, B.W. (1989) In The Use of Crystallography in the Design of Antiviral Agents“, W.G. Laver and G.M. Air, eds. Academic Press, Orlando, Florida.
Bryan, P., Rollence, M., Pantoliano, M., Quill, S., Wood, J., Perna, L., Matthew, J., Hsiao, H. and Poulos, T. (1985) J. Cell. Biochem. Suppl. 9B, 92.
Dao-pin, S., Baase, W. and Matthews, B.W. (1989) Proteins: Structure, Function and Genetics, manuscript submitted.
Grütter, M.G, Gray, T.M., Weaver, L.H., Alber, T., Wilson, K. and Matthews, B.W. (1987) J. Mol. Biol. 197, 315–329.
Kauzmann, W. (1959) Adv. Prot. Chem. 14, 1–63.
Matsumura, M., Becktel, W.J. and Matthews, B.W. (1988) Nature 334, 406–410.
Matsumura, M., Becktel, W.J. and Matthews, B.W. (1988) Nature 334, 406–410.
Matsumura, M., Wozniak, J.A., Daopin, S. and Matthews, B.W. (1989b) J. Biol. Chem., manuscript submitted.
Matthews, B.W. (1987) Biochemistry 26, 6885–6888.
Matthews, B.W., Nicholson, H. and Becktel, W.J. (1987) Proc. Natl. Acad. Sci. USA 84, 6663–6667.
Mitchinson, C. and Baldwin, R.L. (1986) Proteins: Struct. Funct. Genet. 1, 23–33.
Nicholson, H., Becktel, W.J. and Matthews, B.W. (1988) Nature 336, 651–656.
Nicholson, H., Söderlind, E., Tronrud, D.E. and Matthews, B.W. (1989) J. Mol. Biol., submitted.
Pabo, C.O. and Suchanek, E.G. (1986) Biochemistry 25, 5987–5991.
Pace, C.N., Grimsley, G.R., Thomson, J.A. and Barnett, B.J. (1988) J. Biol. Chem. 263, 11820–11825.
Pantoliano, M.W., Ladner, R.C., Bryan, P.N., Rollence, M.L., Wood, J.F. and Poulos, T.L. (1987) Biochemistry 26, 2077–2082.
Perry, L.J. and Wetzel, R. (1984) Science 226, 555–557.
Richardson, J.S. (1981) Adv. Prot. Chem. 34, 167–339.
Sauer, R.T., Hehir, K., Stearman, R.S., Weiss, M.A., Jeitler-Nilsson, A., Suchanek, E.G. and Pabo, C.O. (1986) Biochemistry 25, 5992–5998.
Thornton, J.M. (1981) J. Mol. Biol. 151, 261–287.
Villafranca, J.E., Howell, E.E., Oatley, S.J., Xuong, N-H. and Kraut, J. (1987) Biochemistry 26, 2182–2189.
Wells, J.A. and Powers, D.B. (1986) J. Biol. Chem. 261, 6564–6570.
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Wozniak, J.A. et al. (1990). Crystallographic and Genetic Approaches Toward the Design of Proteins of Enhanced Thermostability. In: Bugg, C.E., Ealick, S.E. (eds) Crystallographic and Modeling Methods in Molecular Design. Springer, New York, NY. https://doi.org/10.1007/978-1-4612-3374-9_6
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DOI: https://doi.org/10.1007/978-1-4612-3374-9_6
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