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Role of ICAM-1 in the Adherence of Human Neutrophils to Human Endothelial Cells In Vitro

  • C. Wayne Smith
  • Steven D. Marlin
  • Robert Rothlein
  • Michael B. Lawrence
  • Larry V. McIntire
  • Donald C. Anderson

Abstract

The adherence of human neutrophils to endothelial cells and protein-coated foreign surfaces in vitro is significantly increased by chemotactic stimulation or exposure of the cells to secretagogues (1–7). The CD11b/ CD18 (Mac-1) heterodimer on the neutrophil’s surface appears to play a role as shown by the inhibitory effect of some monoclonal antibodies reactive with either CD1 lb or CD18 (4,5,8,9). Stimulation of endothelial cells with bacterial endotoxin (LPS) (4,10,11), interleukin-1 (IL-1) (4,12,13), tumor necrosis factor-α (TNF-α) (4,5,12–14), or lymphotoxin (LT) (13) increases the adherence of unstimulated neutrophils. In contrast to the rapid response following chemotactic stimulation of neutrophils, this increase is not evident until > 1 hour after stimulation, and protein synthesis is required. The specific CD11/CD18 heterodimers important to this cytokine-induced adherence have not been defined. We have recently obtained evidence that the CD18-dependent adherence of human neutrophils to cytokine-stimulated endothelial cells involves intercellular adherence molecule-1 (ICAM-1) expressed on the endothelial surface (7). In light of recent evidence that ICAM-1 (15,16) is a ligand for the CD11a/ CD18 (LFA-1) heterodimer (17,18), consideration was given to the possibility that LFA-1 contributes to the adherence of neutrophils to cytokine stimulated endothelial cells.

Keywords

Wall Shear Stress Human Neutrophil Flow Chamber Transendothelial Migration Neutrophil Adherence 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag New York Inc. 1990

Authors and Affiliations

  • C. Wayne Smith
  • Steven D. Marlin
  • Robert Rothlein
  • Michael B. Lawrence
  • Larry V. McIntire
  • Donald C. Anderson

There are no affiliations available

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